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Flow cytometric analysis of HCV Core Protein expression in HCV Genotype 1b-transfected cells. HCV Core Protein Genotype 1b-transfected cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; dashed line histogram) or BD Horizon™ R718 Mouse Anti-HCV Core Protein antibody (Cat. No. 569374/569375; solid line histogram) at 1 µg/test. The fluorescence histogram showing HCV Core Protein expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ R718 Mouse Anti-HCV Core Protein
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
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- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
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Companion Products
The C7-50 monoclonal antibody specifically recognizes the Hepatitis C Virus (HCV) Genotype 1b Core Protein. This antibody binds to a linear epitope within amino acids 21 to 40 that is highly conserved among all HCV genotypes. HCV is a positive single-stranded RNA virus with a genome of 9,600 nucleotides that encodes a single polyprotein. This polyprotein is post-translationally processed into several structural and non-structural proteins required for viral replication. The HCV core protein is a highly basic RNA-binding protein that surrounds the genome to form the viral nucleocapsid. C7-50 has been used for studying HCV in experimental model systems, including HCV gene-transfected cells.
Development References (6)
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Heintges T, zu Putlitz J, Wands JR. Characterization and binding of intracellular antibody fragments to the hepatitis C virus core protein.. Biochem Biophys Res Commun. 1999; 263(2):410-8. (Clone-specific: Immunoprecipitation, Northern blot, Western blot). View Reference
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Kang W, Sung PS, Park SH, et al. Hepatitis C virus attenuates interferon-induced major histocompatibility complex class I expression and decreases CD8+ T cell effector functions.. Gastroenterology. 2014; 146(5):1351-60.e1-4. (Biology). View Reference
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Kannan RP, Hensley LL, Evers LE, Lemon SM, McGivern DR. Hepatitis C virus infection causes cell cycle arrest at the level of initiation of mitosis.. J Virol. 2011; 85(16):7989-8001. (Clone-specific: Flow cytometry, Immunofluorescence, Western blot). View Reference
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Kato N. Genome of human hepatitis C virus (HCV): gene organization, sequence diversity, and variation.. Microb Comp Genomics. 2000; 5(3):129-51. (Biology). View Reference
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Moradpour D, Wakita T, Tokushige K, Carlson RI, Krawczynski K, Wands JR. Characterization of three novel monoclonal antibodies against hepatitis C virus core protein.. J Med Virol. 1996; 48(3):234-41. (Immunogen: ELISA, Immunofluorescence, Radioimmunoassay, Western blot). View Reference
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Yasui K, Wakita T, Tsukiyama-Kohara K, et al. The native form and maturation process of hepatitis C virus core protein.. J Virol. 1998; 72(7):6048-55. (Clone-specific: Immunofluorescence). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.