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      Purified Rat Anti-Mouse Flk-1
      Purified Rat Anti-Mouse Flk-1
      Immunohistochemical staining of mouse Flk-1. Frozen sections of normal mouse spleen were reacted with Purified Rat Anti-Mouse Flk-1 (Cat. No. 550549), then visualized with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and the DAB detection system (Cat. No. 550880). Endothelial cells expressing Flk-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
      Purified Rat Anti-Mouse Flk-1
      Immunohistochemical staining of mouse Flk-1. Frozen sections of normal mouse spleen were reacted with Purified Rat Anti-Mouse Flk-1 (Cat. No. 550549), then visualized with Biotin Goat Anti-Rat Ig (Cat. No. 559286), Streptavidin-HRP (Cat. No. 550946), and the DAB detection system (Cat. No. 550880). Endothelial cells expressing Flk-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
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      BD Pharmingen™
      CD309; Fetal liver kinase 1; Kdr; VEGF Receptor-2; VEGFR-2; AVAS12;
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2a, κ
      Mouse Flk-1 Recombinant Protein
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
      31.25 µg/ml
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      Immunohistochemistry: The Avas 12α1 antibody specific for mouse Flk-1 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and gastrointestinal tract. The antibody stains endothelial cells. The isotype control recommended for use with this antibody is Purified Rat IgG2a κ Isotype Control (Cat. No. 559073). For optimal indirect immunohistochemical staining, the Avas 12α1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Rat Ig (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, Anti-rat Ig HRP Detection Kit (Cat. No. 551013) offers all of the necessary reagents to stain for this antibody.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550549 Rev. 4
      Antibody Details
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      Avas 12α1

      The Avas 12α1 monoclonal antibody specifically binds to fetal liver kinase 1 (Flk-1) which is also known as CD309. Flk-1 is a receptor protein tyrosine kinase which is closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2 or VEGFR2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic and adult tissues. In vivo and in vitro studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells.

      550549 Rev. 4
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550549 Rev.4
      Citations & References
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      View product citations for antibody "550549" on CiteAb

      Development References (8)

      1. Hanahan D. Signaling vascular morphogenesis and maintenance. Science. 1997; 277(5322):48-50. (Biology). View Reference
      2. Kataoka H, Takakura N, Nishikawa S, et al. Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells.. Dev Growth Differ. 1997; 39(6):729-40. (Immunogen). View Reference
      3. Millauer B, Wizigmann-Voos S, Schnurch H, et al. High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis. Cell. 1993; 72(6):835-846. (Biology). View Reference
      4. Nishikawa SI, Nishikawa S, Hirashima M, Matsuyoshi N, Kodama H. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development. 1998; 125(9):1747-1757. (Biology). View Reference
      5. Nishikawa SI, Nishikawa S, Kawamoto H, et al. In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos. Immunity. 1998; 8(6):761-769. (Biology). View Reference
      6. Quinn TP, Peters KG, De Vries C, Ferrara N, Williams LT. Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc Natl Acad Sci U S A. 1993; 90(16):7533-7537. (Biology). View Reference
      7. Shalaby F, Ho J, Stanford WL, et al. A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis. Cell. 1997; 89(6):981-990. (Biology). View Reference
      8. Shalaby F, Rossant J, Yamaguchi TP, et al. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature. 1995; 376(6535):62-66. (Biology). View Reference
      View All (8) View Less
      550549 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.