Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with lipopolysaccharide (LPS) (1 ug/ml, 20-24h, 37°C). The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm™ solution (Cat. No. 554722) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with either Purified IgG1, κ Isotype Control (Cat. No. 554121; Left Plot) or Purified Mouse Anti-Human IDO1 antibody (Cat. No. 568020; Right Plot) at 0.5 ug/test followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Bivariate pseudocolor plots showing the correlated expression of IDO1 (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with lipopolysaccharide (LPS) (1 ug/ml, 20-24h, 37°C). The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm™ solution (Cat. No. 554722) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with either Purified IgG1, κ Isotype Control (Cat. No. 554121; Left Plot) or Purified Mouse Anti-Human IDO1 antibody (Cat. No. 568020; Right Plot) at 0.5 ug/test followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Bivariate pseudocolor plots showing the correlated expression of IDO1 (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
IDO; IDO-1; INDO; indoleamine 2,3-dioxygenase 1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human IDO1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
568020 Rev. 1
Antibody Details
V50-1886
The V50-1886 monoclonal antibody specifically binds to human IDO1, or indoleamine 2, 3-dioxygenase 1, a rate-limiting enzyme in the catabolism of tryptophan. IDO1 is expressed in normal epithelial, endothelial and myeloid cells and in tumor cells. Its expression is induced by interferon gamma and increases in viral and bacterial infections. The reduction of tryptophan and increase in its metabolites that are mediated by IDO1 have critical immunosuppressive and bactericidal effects.
568020 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
568020 Rev.1
Citations & References
View product citations for antibody "568020" on CiteAb
Development References (6)
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Liu H, Shen Z, Wang Z, et al. Increased expression of IDO associates with poor postoperative clinical outcome of patients with gastric adenocarcinoma.. Sci Rep. 2016; 6:21319. (Biology). View Reference
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Nakamura T1, Shima T, Saeki A, et al. Expression of indoleamine 2, 3-dioxygenase and the recruitment of Foxp3-expressing regulatory T cells in the development and progression of uterine cervical cancer.. 2007; 98(6):874-881. (Biology). View Reference
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Takikawa O1, Kuroiwa T, Yamazaki F, Kido R.. Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity.. J Biol Chem. 1988; 263(4):2041-2048. (Biology). View Reference
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Théate I, van Baren N, Pilotte L, et al. Extensive profiling of the expression of the indoleamine 2,3-dioxygenase 1 protein in normal and tumoral human tissues.. Cancer Immunol Res. 2015; 3(2):161-72. (Biology). View Reference
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Zamorina SA, Timganova VP, Bochkova MS, Khramtsov PV, Raev MB. Effect of pregnancy-specific β1-glycoprotein on indoleamine-2,3-dioxygenase activity in human monocytes.. Dokl Biol Sci. 2016; 469(1):206-8. (Biology). View Reference
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van Baren N, Van den Eynde BJ. Tryptophan-degrading enzymes in tumoral immune resistance.. Front Immunol. 2015; 6:34. (Biology). View Reference
568020 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.