PE Rat Anti-Pig γδ T Lymphocytes

BD Pharmingen™ PE Rat Anti-Pig γδ T Lymphocytes

Name: PE Rat Anti-Pig γδ T Lymphocytes
Brand: BD Pharmingen™
SKU: 561486

Clone MAC320

(RUO)

Flow cytometric analysis of γδ T cell expression on pig peripheral blood lymphocytes. Pig whole blood was stained with PE Rat Anti-Pig γδ T lymphocytes (Cat. No. 561486; solid line histogram) or with a PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.

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Product Details

Brand: BD Pharmingen™

Reactivity: Pig (QC Testing)

Isotype: Rat PVG, also known as HO, Black Hooded IgG2a, κ

Immunogen: Null T cells purified from the blood of young pigs

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10680274

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Lysing Buffer RUO

Size 100 mL Cat No. 555899

PE Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553930

561486 Rev. 1

Antibody Details

MAC320

The MAC320 antibody monoclonal antibody specifically binds to the 270- and 280-kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes.

561486 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

561486 Rev.1

Citations & References

View product citations for antibody "561486" on CiteAb

Development References (5)

Binns RM, Duncan IA, Powis SJ, Hutchings A, Butcher GW. Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies. Immunology. 1992 October; 77(2):219-227. (Immunogen: Cytotoxicity, Immunoprecipitation). View Reference
Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). View Reference
Binns RM. The Null/gamma delta TCR+ T cell family in the pig. Vet Immunol Immunopathol. 1994 October; 43(1-3):69-77. (Biology). View Reference
Davis WC, Zuckermann FA, Hamilton MJ, et al. Analysis of monoclonal antibodies that recognize gamma delta T/null cells. Vet Immunol Immunopathol. 1998 January; 60(3-4):305-316. (Biology). View Reference
Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology). View Reference

View All (5)

561486 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.