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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
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The 3A4 monoclonal antibody (also known as, 3A4/Clec4d) specifically recognizes mouse CD368 which is also known as C-type lectin domain family 4 member D (Clec4d) and Macrophage C-type lectin (MCL). CD368 (Clec4d) is a single pass type II transmembrane glycoprotein that is encoded by Clec4d which belongs to the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CD368 (Clec4d) is expressed by myeloid cells, including monocytes, macrophages, and neutrophils as well as by some dendritic cells. This calcium dependent lectin is involved in innate immune sensing of pathogen-associated molecular patterns (PAMPs) such as mycobacterial trehalose 6,6'-dimycolate. It functions as an endocytic receptor that may be involved in antigen clearance as well as antigen presentation to T cells including T-helper 1 (Th1)- and Th17-like T cells. The expression of CD368 (Clec4d) may be upregulated in response to various microbial stimuli including TLR agonists.
Development References (3)
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Hupfer T, Schick J, Jozefowski K, Voehringer D, Ostrop J, Lang R. Stat6-Dependent Inhibition of Mincle Expression in Mouse and Human Antigen-Presenting Cells by the Th2 Cytokine IL-4.. Front Immunol. 2016; 7:423. (Clone-specific: Flow cytometry). View Reference
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Kerscher B, Dambuza IM, Christofi M, et al. Signalling through MyD88 drives surface expression of the mycobacterial receptors MCL (Clecsf8, Clec4d) and Mincle (Clec4e) following microbial stimulation.. Microbes Infect. 2016; 18(7-8):505-9. (Clone-specific: Flow cytometry). View Reference
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Kerscher B, Wilson GJ, Reid DM, et al. Mycobacterial receptor, Clec4d (CLECSF8, MCL), is coregulated with Mincle and upregulated on mouse myeloid cells following microbial challenge.. Eur J Immunol. 2016; 46(2):381-9. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.