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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The CA2 monoclonal antibody specifically recognizes Cationic amino acid transporter-1 (CAT1), which is also known as High affinity cationic amino acid transporter 1 (SLC7A1). CAT1 (SLC7A1) is a ~ 67 kDa ubiquitously expressed glycosylated transmembrane protein encoded by SLC7A1 which belongs to cationic amino acid transporters (CAT) subfamily within the solute carrier (SLC) 7 family. In non-hepatic tissues, this high-affinity, low capacity permease mediates the bidirectional transport of cationic amino acids (arginine, lysine and ornithine) by facilitated diffusion across membranes for cellular metabolism. CAT1 (SLC7A1) provides key support for T cell growth and proliferation and is also known as a potential ecotropic retroviral leukemia receptor. CAT1 (SLC7A1) protein is highly expressed in certain cancers, such as breast, colorectal and chronic lymphocytic leukemia, including inflammatory and autoimmune diseases. This protein reportedly serves as a promising regulator of adaptive immune responses in tumor immunity or inflammation.
Development References (8)
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Abdelmagid SA, Rickard JA, McDonald WJ, Thomas LN, Too CK. CAT-1-mediated arginine uptake and regulation of nitric oxide synthases for the survival of human breast cancer cell lines.. J Cell Biochem. 2011; 112(4):1084-92. (Biology). View Reference
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Closs EI, Simon A, Vékony N, Rotmann A. Plasma membrane transporters for arginine.. J Nutr. 2004; 134(10 Suppl):2752S-2759S; discussion 2765S-2767S. (Biology). View Reference
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Jungnickel KEJ, Parker JL, Newstead S. Structural basis for amino acid transport by the CAT family of SLC7 transporters.. Nat Commun. 2018; 9(1):550. (Biology). View Reference
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Lu Y, Hao C, Yu S, et al. Cationic amino acid transporter-1 (CAT-1) promotes fibroblast-like synoviocyte proliferation and cytokine secretion by taking up L-arginine in rheumatoid arthritis.. Arthritis Res Ther. 2022; 24(1):234. (Biology). View Reference
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Okita K, Hara Y, Okura H, et al. Antitumor effects of novel mAbs against cationic amino acid transporter 1 (CAT1) on human CRC with amplified CAT1 gene.. Cancer Sci. 2021; 112(2):563-574. (Immunogen). View Reference
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Verrey F, Closs EI, Wagner CA, Palacin M, Endou H, Kanai Y. CATs and HATs: the SLC7 family of amino acid transporters.. Pflugers Arch. 2004; 447(5):532-42. (Biology). View Reference
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Werner A, Amann E, Schnitzius V, et al. Induced arginine transport via cationic amino acid transporter-1 is necessary for human T-cell proliferation.. Eur J Immunol. 2016; 46(1):92-103. (Biology). View Reference
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Werner A, Pieh D, Echchannaoui H, et al. Cationic Amino Acid Transporter-1-Mediated Arginine Uptake Is Essential for Chronic Lymphocytic Leukemia Cell Proliferation and Viability.. Front Oncol. 2019; 9:1268. (Biology: Cytotoxicity, Flow cytometry, Immunohistochemistry, Inhibition). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.