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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
The 14.27 monoclonal antibody specifically recognizes CD38, which is also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 or ADP-ribosyl cyclase 1 (ADPRC 1). CD38 a 45 kDa type II transmembrane glycoprotein that is encoded by Cd38 (CD38 molecule). CD38 is variably expressed on early hematopoietic precursors and leucocytes including immature and mature B cells as well as thymocytes, T cells, NK cells, monocytes, and macrophages. It is also expressed on non-hematopoietic cells such as epithelial cells, astrocytes, hepatic stellate cells (HSCs), and Kupffer cells. CD38 catalyzes the synthesis and hydrolysis of cyclic adenosine diphosphate ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) which can function as secondary messengers involved in calcium mobilization. The 14.27 antibody can reportedly stimulate increased cytosolic Ca2+ levels, IL-6 production and increased expression of the adhesion molecules, VCAM-1, N-CAM, and ICAM-1 by HSCs.
Development References (3)
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Bath NM, Ding X, Wilson NA, et al. Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model.. PLoS One. 2019; 14(2):e0211865. (Clone-specific: Flow cytometry). View Reference
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March S, Graupera M, Rosa Sarrias M, et al. Identification and functional characterization of the hepatic stellate cell CD38 cell surface molecule.. Am J Pathol. 2007; 170(1):176-87. (Immunogen: Calcium Flux, Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Pearse DD, Bastidas J, Izabel SS, Ghosh M. Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.. Int J Mol Sci. 2018; 19(9):E2550. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.