Multicolor flow cytometric analysis of Ebf-1 expression in mouse bone marrow cells and splenocytes. C57BL/6 mouse bone marrow cells (Top Panels) and splenic leucocytes (Bottom Panels) were surface stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat No. 553092/561880). The cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panels) or PE Mouse Anti-Ebf1 antibody (Cat. No. 565494). The two-color contour plots showing the correlated expression of Ebf1 (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
.png){kind=spacer}
Multicolor flow cytometric analysis of Ebf-1 expression in mouse bone marrow cells and splenocytes. C57BL/6 mouse bone marrow cells (Top Panels) and splenic leucocytes (Bottom Panels) were surface stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat No. 553092/561880). The cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panels) or PE Mouse Anti-Ebf1 antibody (Cat. No. 565494). The two-color contour plots showing the correlated expression of Ebf1 (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
BD Pharmingen™
Ebf1; Early B-cell factor 1; Ebf; Coe1; O/E-1; OE-1; Olf-1; Olf1
Mouse (QC Testing)
Mouse IgG1, κ
Mouse EBF1 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739263
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Transcription Factor Buffer Set RUO
Size
100 Tests
Cat No.
562574
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
APC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553092
APC Rat Anti-Mouse CD45R/B220 RUO
Size
25 µg
Cat No.
561880
565494 Rev. 1
Antibody Details
T26-818
The T26-818 monoclonal antibody specifically binds to Early B-cell Factor 1 (Ebf1) which is also known as, Transcription factor COE1, or Olfactory 1 (Olf-1/Olf1). Ebf1 belongs to the Collier/Olf1/EBF (COE) family of transcription factors. It is involved in early B cell development in the mouse and human. It regulates the activation of a series of B cell-specific genes and also represses the activation of non-B lineage genes. Ebf1 is highly expressed in B cell progenitors in the bone marrow and it has also been detected in a series of cell lines within the B-cell lineage. Ebf1 and other members of the EBF family of transcription factors also play roles in the migration, commitment, and differentiation of other cell types including those involved in neurogenesis and adipogenesis.
565494 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565494 Rev.1
Citations & References
View product citations for antibody "565494" on CiteAb
Development References (4)
-
Boller S, Grosschedl R. The regulatory network of B-cell differentiation: a focused view of early B-cell factor 1 function. Immunol Rev. 2014; 261(1):102-115. (Biology). View Reference
-
Gao H, Mejhert N, Fretz JA. Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue. Cell Metab. 2014; 19(6):981-992. (Biology). View Reference
-
Hagman J, Ramírez J, Lukin K. B lymphocyte lineage specification, commitment and epigenetic control of transcription by early B cell factor 1. Curr Top Microbiol Immunol. 2012; 356:17-38. (Biology). View Reference
-
Åhsberg J, Ungerbäck J, Strid T, et al. Early B-cell factor 1 regulates the expansion of B-cell progenitors in a dose-dependent manner. J Biol Chem. 2013; 288(46):33449-334461. (Biology). View Reference
565494 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.