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      PE Mouse Anti-Mouse CD28
      PE Mouse Anti-Mouse CD28
      Two-color flow cytometric analysis of Mouse CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either PE Mouse IgG2b, κ Isotype Control (Cat. No.559529; Left Plot) or PE Mouse Anti-CD28 antibody (Cat. No. 566815; Right Plot) at 0.5 μg/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE Mouse Anti-Mouse CD28
      Two-color flow cytometric analysis of Mouse CD28 expression on mouse splenocytes. BALB/c mouse spleen cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either PE Mouse IgG2b, κ Isotype Control (Cat. No.559529; Left Plot) or PE Mouse Anti-CD28 antibody (Cat. No. 566815; Right Plot) at 0.5 μg/test. A two-color flow cytometric dot plot showing the correlated expression of CD3e versus CD28 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      Cd28; T-cell-specific surface glycoprotein CD28
      Mouse (QC Testing)
      Mouse IgG2b, κ
      Mouse CD28
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2869882
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566815 Rev. 1
      Antibody Details
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      E18

      The E18 monoclonal antibody specifically recognizes CD28 that is encoded by Cd28 (CD28 antigen) which belongs to the immunoglobulin gene superfamily. CD28 is a ~45 kDa type I transmembrane glycoprotein that can be expressed as a disulfide-linked homodimer. The extracellular region of CD28 contains an IgV-like domain which is followed by a transmembrane region and a cytoplasmic domain. CD28 is expressed on thymocytes, most peripheral CD4+ T cells and CD8+ T cells, NK cells, and plasmacytoid dendritic cells. CD28 functions as a costimulatory receptor upon binding ligands that include CD80 (B7-1) and CD86 (B7-2) that are expressed on antigen presenting cells and B cells. CD28-mediated costimulation augments the proliferation and cytokine production of activated T and NK cells, provides a costimulatory signal for CTL induction, and promotes T cell survival. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses. The E18 antibody reportedly blocks the binding of B7 antigens to CD28 and can costimulate T cell activation and proliferation. Spleen cells from a CD28-deficient mouse that was immunized with mouse CD28 were used to generate the hybridoma that secretes the E18 antibody.

              

      566815 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      566815 Rev.1
      Citations & References
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      View product citations for antibody "566815" on CiteAb

      Development References (3)

      1. Beyersdorf N, Ding X, Blank G, Dennehy KM, Kerkau T, Hünig T. Protection from graft-versus-host disease with a novel B7 binding site-specific mouse anti-mouse CD28 monoclonal antibody.. Blood. 2008; 112(10):4328-36. (Clone-specific: Blocking, Functional assay). View Reference
      2. Dennehy KM, Elias F, Zeder-Lutz G, et al. Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.. J Immunol. 2006; 176(10):5725-9. (Immunogen: Flow cytometry). View Reference
      3. Macal M, Tam MA, Hesser C, et al. CD28 Deficiency Enhances Type I IFN Production by Murine Plasmacytoid Dendritic Cells. J Immunol. 2016; 196(4):1900-1909. (Clone-specific: Flow cytometry). View Reference
      566815 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.