Flow cytometric analysis of Platelet GPVI expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Platelet GPVI antibody (Cat. No. 565241; solid line histogram). The fluorescence histogram showing GPVI expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
.png){kind=spacer}
Flow cytometric analysis of Platelet GPVI expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human Platelet GPVI antibody (Cat. No. 565241; solid line histogram). The fluorescence histogram showing GPVI expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Glycoprotein 6; GP6; Platelet glycoprotein VI; Platelet collagen receptor
Human (QC Testing)
Mouse IgG1, κ
Glycoprotein VI Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
AB_2739131
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
565241 Rev. 1
Antibody Details
HY101
The HY101 monoclonal antibody specifically binds to a collagen receptor, Platelet Glycoprotein VI (GPVI or GP6). GPVI is a 58-kD platelet membrane glycoprotein that belongs to the immunoglobulin superfamily. Upon blood vessel wall injury and consequent endothelial cell damage, platelets collect upon the exposed collagen fibers present in the subendothelial matrix. The platelet and collagen interaction leads to activated platelet aggregation and thrombus formation. Although normally serving a protective function in clot formation that limits blood loss, the platelet GPVI/collagen interaction may also play a role in myocardial infarction and stroke which are associated with the rupture of atherosclerotic plaques.
565241 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565241 Rev.1
Citations & References
View product citations for antibody "565241" on CiteAb
Development References (4)
-
Chen H, Locke D, Liu Y, Liu C, Kahn ML. The platelet receptor GPVI mediates both adhesion and signaling responses to collagen in a receptor density-dependent fashion. J Biol Chem. 2002; 277(4):3011-3019. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay, Western blot). View Reference
-
Ezumi Y, Uchiyama T, Takayama H.. Molecular cloning, genomic structure, chromosomal localization, and alternative splice forms of the platelet collagen receptor glycoprotein VI. Biochem Biophys Res Commun. 2000; 277(1):27-36. (Biology). View Reference
-
Hermans C, Wittevrongel C, Thys C, Smethurst PA, Van Geet C, Freson K. A compound heterozygous mutation in glycoprotein VI in a patient with a bleeding disorder. J Thromb Haemost. 2009; 7(8):1356-1363. (Clone-specific: Flow cytometry). View Reference
-
Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood. 2000; 96(5):1798-1807. (Biology). View Reference
565241 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.