Flow cytometric analysis of EGF Receptor expression on human epidermoid carcinoma cells. A-431 cells (ATCC CRL-1555) were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human EGF Receptor antibody (Cat. No. 566778; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing EGF Receptor expression (or Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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Flow cytometric analysis of EGF Receptor expression on human epidermoid carcinoma cells. A-431 cells (ATCC CRL-1555) were stained with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human EGF Receptor antibody (Cat. No. 566778; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing EGF Receptor expression (or Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
EGFR; ERBB; ERBB1; HER1; mENA; PIG61
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human EGF Receptor extracellular domain Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
1956
AB_2869861
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
566778 Rev. 1
Antibody Details
EMab-134
The EMab-134 monoclonal antibody specifically recognizes the epidermal growth factor receptor (EGF-R). The EGF-R is a transmembrane glycoprotein of approximately 170 kDa that is expressed on most cells. It consists of a glycosylated extracellular domain which binds to epidermal growth factor (EGF), a transmembrane domain, and an intracellular domain with tyrosine-kinase activity essential for signal transduction. The epitope recognized by EMab-134 is an extracellular peptide that is not affected by glycosylation. The EGF-R plays an important role in the growth and differentiation of many cellular types. Transforming growth factor α (TGFα), vaccinia virus growth factor, and related growth factors can also bind to and signal through the EGF-R.
566778 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566778 Rev.1
Citations & References
View product citations for antibody "566778" on CiteAb
Development References (5)
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Itai S, Yamada S, Kaneko MK, Chang YW, Harada H, Kato Y. Establishment of EMab-134, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody for Detecting Squamous Cell Carcinoma Cells of the Oral Cavity. Monoclon Antib Immunodiagn Immunother. 2017; 36(6):272-281. (Immunogen: ELISA, Flow cytometry, Immunohistochemistry, Western blot). View Reference
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Kaneko MK, Yamada S, Itai S, et al. Elucidation of the critical epitope of an anti-EGFR monoclonal antibody EMab-134. Biochem Biophys Rep. 2018; 14:54-57. (Clone-specific: Flow cytometry, Immunohistochemistry, Western blot). View Reference
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Kovacs E, Zorn JA, Huang Y, Barros T, Kuriyan J. A structural perspective on the regulation of the epidermal growth factor receptor.. Annu Rev Biochem. 2015; 84:739-64. (Biology). View Reference
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Leahy DJ. Structure and function of the epidermal growth factor (EGF/ErbB) family of receptors.. Adv Protein Chem. 2004; 68:1-27. (Biology). View Reference
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Messa C, Russo F, Notarnicola M, Di Leo A. Demonstration of epidermal growth factor receptor in colorectal adenocarcinoma by enzyme immunoassay. Digestion. 1994; 55(2):103-107. (Biology). View Reference
566778 Rev. 1
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.