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PE Mouse Anti-Human CX3CL1 (Fractalkine)
Flow cytometric analysis of CX3CL1 expression on wild type (Left Panel) or Crispr/Cas9 knockout (Right Panel) HepG2 cells. Wild type or knockout human HepG2 cells were detached with Accutase (Cat. No. 561527) and stained with either Mouse Anti-Human CX3CL1 antibody (Cat. No. 566905; solid line histogram) or PE mIgG1, κ Isotype Control (Cat. No. 556650; dashed line histogram). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of live cells. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Crispr/Cas9 knockout HepG2 was generated by Synthego Corp. Knockout HepG2 cells were bulk sorted and sorted cells were expanded by culturing before staining. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human CX3CL1 (Fractalkine)
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