PE Mouse Anti-Human CD206

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Mouse Anti-Human CD206

Name: PE Mouse Anti-Human CD206
Brand: BD Pharmingen™
SKU: 555954

Clone 19.2

(RUO)

GM-CSF stimulated (3 days) peripheral blood mononuclear cells (gated on large cell population) analyzed on a FACScan (BDIS, San Jose, CA)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Macrophage mannose receptor; MMR; MRC1; CLEC13D

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human Placental Macrophage Mannose Receptor

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VII 70318

Entrez Gene ID: 4360

RRID: AB_396250

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

PE Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555749

555954 Rev. 8

Antibody Details

19.2

The 19.2 monoclonal antibody specifically binds to CD206 which is also known as the macrophage mannose receptor (MMR) or C-type lectin domain family 13 member D (CLEC13D). CD206 is a type I transmembrane glycoprotein of approximately 162 kDa which binds to glycoconjugates containing mannose, fucose, or N-acetylglucosamine. These carbohydrates are present on the surface of many microorganisms and enable the macrophage to bind to microorganisms through the MMR and internalize them during the process of phagocytosis. CD206 is expressed on human macrophages, endothelial cells, and cultured dendritic cells. It is not detected on resting monocytes. Mannose receptor expression is upregulated on peripheral blood mononuclear cells following 3 day incubation with GM-CSF.

555954 Rev. 8

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

555954 Rev.8

Citations & References

Development References (2)

Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
Pontow SE, Kery V, Stahl PD. Mannose receptor. Int Rev Cytol. 1992; 137(B):221-244. (Clone-specific). View Reference

555954 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.