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PE Mouse Anti-Human CD140a (PDGFRα)
PE Mouse Anti-Human CD140a (PDGFRα)
Flow cytometric analysis of CD140a (PDGFRα) expression on human MG-63 cells. Cells from the human MG-63 (Osteosarcoma, ATCC CRL-1427) cell line were stained with BD PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or with BD PE Mouse Anti-Human CD140a (PDGFRα) antibody (Cat. No. 567950/567951; solid line histogram). The histogram showing CD140a (PDGFRα) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable MG-63 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of CD140a (PDGFRα) expression on human MG-63 cells. Cells from the human MG-63 (Osteosarcoma, ATCC CRL-1427) cell line were stained with BD PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or with BD PE Mouse Anti-Human CD140a (PDGFRα) antibody (Cat. No. 567950/567951; solid line histogram). The histogram showing CD140a (PDGFRα) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable MG-63 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
CD140a; PDGFRA; PDGF Receptor alpha; PDGF-R-alpha; PDGFRα; PDGFR2
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human PDGF-RA Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
VI E022
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
567950 Rev. 1
Antibody Details
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16A1

The 16A1 monoclonal antibody specifically recognizes human CD140a which is also known as human platelet derived growth factor receptor α (PDGFRα). CD140a (PDGFRα) is an ~170 kDa single pass type I transmembrane glycoprotein that is encoded by PDGFRA (platelet derived growth factor receptor alpha) which belongs to the receptor tyrosine kinase (RTK) class III family. This receptor is comprised of an N-terminal extracellular domain with five IgC2-like domains, and a transmembrane sequence followed by a cytoplasmic region with a split tyrosine kinase domain. CD140a (PDGFRα) is expressed on a variety of cell types including platelets, monocytes, fibroblasts, endothelial cells, smooth muscle cells, glial cells, chondrocytes as well as numerous tumor cell types. PDGFRα dimerizes and autophosphorylates when binding to homodimeric or heterodimeric platelet-derived growth factors (PDGF) comprised of disulfide-bonded A, B, or C (but not D) isoforms, ie, PDGF-AA, PDGF-AB, PDGF-BB, and PDGF-CC. PDGFRα can also heterodimerize with platelet derived growth factor receptor β (PDGFRβ, also known as CD140b) and autophosphorylate upon binding to PDGF-AB, PDGF-BB, and possibly PDGF-CC and PDGF-DD. Homodimerized CD140b (PDGFRβ) is activated by PDGF-BB and PDGF-DD. PDGF ligands stimulate the growth, proliferation, migration, and survival of PDGF Receptor-positive normal cells as well as tumor cells. CD140a (PDGFRα) plays key roles in multiple processes including embryonic development, wound healing, and the regulation of platelet activation.

        

567950 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
567950 Rev.1
Citations & References
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View product citations for antibody "567950" on CiteAb

Development References (3)

  1. Demoulin JB, Essaghir A. PDGF receptor signaling networks in normal and cancer cells. Cytokine Growth Factor Rev. 2014; 25(3):273-283. (Biology). View Reference
  2. Hart CE, Bowen-Pope DF. CD140a and b (PDGRα and β) Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:739-741.
  3. Miyazaki S, Sugawara H, Tamura T. Cross-lineage (Blind Panel) study and human leucocyte differentiation antigen database. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:3-20.
567950 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.