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      PE-Cy7 Mouse Anti-Human IL-22
      PE-Cy7 Mouse Anti-Human IL-22
      Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, 50 ng/ml) and Ionomycin (Sigma, 500 ng/ml) in the presence of BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 (Cat. No. 564724) and either PE-Cy7 Mouse IgG2a, κ Isotype Control (Cat. No. 560906; Left Plot) or PE-Cy7 Mouse anti-Human IL-22 antibody (Cat. No. 567579/567567; Right Plot) at 0.03 µg/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE-Cy7 Mouse Anti-Human IL-22
      Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, 50 ng/ml) and Ionomycin (Sigma, 500 ng/ml) in the presence of BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 (Cat. No. 564724) and either PE-Cy7 Mouse IgG2a, κ Isotype Control (Cat. No. 560906; Left Plot) or PE-Cy7 Mouse anti-Human IL-22 antibody (Cat. No. 567579/567567; Right Plot) at 0.03 µg/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      interleukin-22; IL-10-related T-cell-derived inducible factor; IL-TIF; ILTIF; IL-22a, ilTIFa; Iltif; TIFa
      Human (QC Testing)
      Mouse BALB/c IgG2a, κ
      Human IL-22 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036).
      7. An isotype control should be used at the same concentration as the antibody of interest.
      8. PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from PE may be observed. Therefore, we recommend that individual compensation controls be performed for every PE-Cy7 conjugate. PE-Cy7 is optimized for use with a single argon ion laser emitting 488-nm light, and there is no significant overlap between PE-Cy7 and FITC emission spectra. When using dual-laser cytometers, which may directly excite both PE and Cy7, we recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
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      567579 Rev. 1
      Antibody Details
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      MH22B2

      The MH22B2 monoclonal antibody specifically recognizes human Interleukin-22 (IL-22), which is encoded by the IL22 gene. Cross-reactivity of MH22B2 mAb to mouse IL-22 (which shares 79% amino acid identity with human IL-22) has been observed by ELISA and by flow cytometry of HEK293 cells transfected with mouse IL-22 and TH17-differentiated CD4-positive T cells from C57BL/6 mice. IL-22 (with or without other cytokines) is secreted by many T-cell and innate-lymphoid-cell populations. Evidence that IL-22 plays a key role in mucosal immunity includes the restricted expression of the alpha subunit of the heterodimeric IL-22 receptor, called IL-22R1, on epithelial cells and cells of epithelial origin. At epithelial surfaces, IL-22 elicits antimicrobial defenses and maintains epithelial integrity. Alternatively, uncontrolled IL-22 production can result in certain inflammatory disorders. Regulation of IL-22 expression is complex, involving other cytokines (eg, IL-6, IL-23, and TGF-β) and many transcription factors, (eg, AHR, c-Maf, STAT3, RORɤT, BATF, and others).

      567579 Rev. 1
      Format Details
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      PE-Cy7
      PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE-Cy7
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      781 nm
      567579 Rev.1
      Citations & References
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      View product citations for antibody "567579" on CiteAb

      Development References (8)

      1. Colonna M. Interleukin-22-producing natural killer cells and lymphoid tissue inducer-like cells in mucosal immunity.. Immunity. 2009; 31(1):15-23. (Biology). View Reference
      2. Le-Thi-Phuong T, Dumoutier L, Renauld JC, Van Snick J, Coutelier JP. Divergent roles of IFNs in the sensitization to endotoxin shock by lactate dehydrogenase-elevating virus.. Int Immunol. 2007; 19(11):1303-11. (Clone-specific: ELISA). View Reference
      3. Martin B, Hirota K, Cua DJ, Stockinger B, Veldhoen M. Interleukin-17-producing gammadelta T cells selectively expand in response to pathogen products and environmental signals.. Immunity. 2009; 31(2):321-30. (Clone-specific: Flow cytometry). View Reference
      4. Rutz S, Eidenschenk C, Ouyang W. IL-22, not simply a Th17 cytokine.. Immunol Rev. 2013; 252(1):116-32. (Biology). View Reference
      5. Suurmond J, Habets KL, Dorjée AL, Huizinga TW, Toes RE. Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1β. J Immunol. 2016; 164(11):4473-4481. (Biology). View Reference
      6. Trifari S, Kaplan CD, Tran EH, Crellin NK, Spits H. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells.. Nat Immunol. 2009; 10(8):864-71. (Biology). View Reference
      7. Veldhoen M, Hirota K, Westendorf AM, et al. The aryl hydrocarbon receptor links TH17-cell-mediated autoimmunity to environmental toxins.. Nature. 2008; 453(7191):106-9. (Immunogen: Flow cytometry). View Reference
      8. Zenewicz LA. IL-22: There Is a Gap in Our Knowledge.. Immunohorizons. 2018; 2(6):198-207. (Biology). View Reference
      View All (8) View Less
      567579 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.