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BUV737 Mouse Anti-Human CD83
Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/ml of Recombinant Human IL-4 (Cat. No. 554605), 20 ng/ml Recombinant Human TNF (Cat. No. 554618), and 20 ng/ml Recombinant Human GM-CSF (Cat. No. 550068) for 7 days at 37°C. The cells were then stained with either BD Horizon™ BUV737 Mouse IgG1, κ Isotype Control (Cat. No. 564299; dashed line histogram) or BD Horizon BUV737 Mouse Anti-Human CD83 antibody (Cat. No. 564441; solid line histogram). Fluorescence histograms showing CD83 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
BD Horizon™ BUV737 Mouse Anti-Human CD83
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