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      APC Mouse Anti-Human CD133
      APC Mouse Anti-Human CD133
      CD133 expression on retinoblastoma cell line and Human peripheral blood mononuclear cells (PBMC). Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 565381; dashed line histogram) or APC Mouse Anti-Human CD133 antibody (Cat. No. 567909/567910 solid line histogram) at 0.25 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with BD Horizon™ CD34 BV421 antibody (Cat. No. 663981) and with either APC Mouse IgG2b, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) peripheral blood lymphocytes (PBL). Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and with either APC Mouse IgG2b, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.25 μg/test followed by BD Via-Probe™ Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD45 was similarly derived for viable (7-AAD-negative) PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      APC Mouse Anti-Human CD133
      CD133 expression on retinoblastoma cell line and Human peripheral blood mononuclear cells (PBMC). Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either APC Mouse IgG2b, κ Isotype Control (Cat. No. 565381; dashed line histogram) or APC Mouse Anti-Human CD133 antibody (Cat. No. 567909/567910 solid line histogram) at 0.25 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with BD Horizon™ CD34 BV421 antibody (Cat. No. 663981) and with either APC Mouse IgG2b, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) peripheral blood lymphocytes (PBL). Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and with either APC Mouse IgG2b, κ Isotype Control (Left Plot) or APC Mouse Anti-Human CD133 antibody (Right Plot) at 0.25 μg/test followed by BD Via-Probe™ Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD45 was similarly derived for viable (7-AAD-negative) PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      PROM1; PROML1; prominin-1; AC133; CORD12; MCDR2; MSTP061; RP41; STGD4
      Human (QC Testing)
      Mouse BALB/c IgG2b, κ
      Human full-length CD133 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      8842
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      567909 Rev. 1
      Antibody Details
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      293C3

      The 293C3 monoclonal antibody specifically recognizes CD133 which is also known as Prominin-like protein 1 (PROML1), Prominin-1 (PROM1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), or AC133 antigen. CD133 is an ~120 kDa five-transmembrane, glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. CD133 is expressed on some cells found in different tissues including the bone marrow, cord and peripheral blood, placenta, liver, pancreas, kidney, lung, retina, brain and heart. It is expressed on a variety of cell types including hematopoietic stem cells and progenitor cells, neural stem cells, developing epithelial cells, precursor endothelial cells, and retinal cells. CD133 is expressed on some cancer cells found in leukemias, melanoma and retinoblastoma. It may serve as a cancer stem cell marker in a number of brain tumors, melanoma, colon cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, and prostate cancer. A mutation in PROM1 has been associated with a form of human retinal degeneration. The 293C3 antibody recognizes a different epitope than the human CD133-specific W6B3C1 antibody.

      567909 Rev. 1
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      567909 Rev.1
      Citations & References
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      View product citations for antibody "567909" on CiteAb

      Development References (5)

      1. Bühring HK, Marzer A, Lammers R, Wissinger B. CD133 cluster report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:622-623.
      2. Koerner SP, André MC, Leibold JS, et al. An Fc-optimized CD133 antibody for induction of NK cell reactivity against myeloid leukemia.. Leukemia. 2017; 31(2):459-469. (Clone-specific: Blocking, Flow cytometry). View Reference
      3. Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W, Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial cellular adhesion molecule, a cell surface antigen expressed in early steps of erythropoiesis.. Exp Hematol. 2002; 30(6):537-45. (Immunogen: Flow cytometry). View Reference
      4. Maw MA, Corbeil D, Koch J, et al. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.. Hum Mol Genet. 2000; 9(1):27-34. (Biology). View Reference
      5. Miraglia SJ, Buck D. CD133 (AC133). In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:870-872.
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      567909 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.