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Alexa Fluor® 488 Mouse IgG1 κ Isotype Control
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
BD Phosflow™ Alexa Fluor® 488 Mouse IgG1 κ Isotype Control
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