Skip to main content Skip to navigation
Purified Mouse Anti-human Clusterin
Purified Mouse Anti-human Clusterin

Immunohistochemical analysis of clusterin expression in human tonsil. Formalin-fixed, paraffin-embedded sections of human tonsil were stained Purified Mouse Anti-human Clusterin (Cat. No. 552886), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat. No. 550337) and Streptavidin HRP (Cat. No. 550946). Dendritic cells in the follicle and basement membrane of epithelial cells and fibrocytes expressed clusterin. Magnification 20X.

Immunohistochemical analysis of clusterin expression in human tonsil. Formalin-fixed, paraffin-embedded sections of human tonsil were stained Purified Mouse Anti-human Clusterin (Cat. No. 552886), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat. No. 550337) and Streptavidin HRP (Cat. No. 550946). Dendritic cells in the follicle and basement membrane of epithelial cells and fibrocytes expressed clusterin. Magnification 20X.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Tested During Development)
AB_394515
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: Purified Mouse Anti-human Clusterin was tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. The tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878). For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Detection System (Cat. No. 550880). More conveniently, the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011), which contains Biotin Goat Anti-Mouse Ig (Multiple Adsorption), antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining. A detailed protocol of the immunohistochemical procedure is available at  http://www.bdbiosciences.com/us/s/resources.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552886 Rev. 2
Antibody Details
Down Arrow Up Arrow
E5

The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.

552886 Rev. 2
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
552886 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Murphy BF, Kirszbaum L, Walker ID, d'Apice AJ. SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis. J Clin Invest. 1988; 81(6):1858-1864. (Clone-specific: Western blot). View Reference
  2. Rosenberg ME, Silkensen J. Clusterin: physiologic and pathophysiologic considerations. Int J Biochem Cell Biol. 1995; 27(7):633-645. (Clone-specific). View Reference
  3. Wellmann A, Thieblemont C, Pittaluga S, et al. Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas. Blood. 2000; 96(2):398-404. (Clone-specific: Western blot). View Reference
552886 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.