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Purified Mouse Anti-Ufd1L
Purified Mouse Anti-Ufd1L

Western blot analysis of Ufd1L on a mouse macrophage cell lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of mouse anti-Ufd1L antibody.

Purified Mouse Anti-Ufd1L

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis of Ufd1L on a mouse macrophage cell lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of mouse anti-Ufd1L antibody.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat (Tested in Development)
Mouse IgG1
Mouse Ufd1L aa. 120-241
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
40 kDa
250 µg/ml
AB_399070
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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19/Ufd1L

Microdeletions of the chromosomal region 22q11.2 are associated with defects in cardiac and craniofacial development that are referred to as the CATCH 22 phenotype and include DiGeorge syndrome, the velo-cardio-facial syndrome, and the Opitz GBBB syndrome. Deleted in CATCH 22 patients, UFD1L encodes the human homolog of the yeast ubiquitin fusion degradation 1 protein (UFD1p), an essential component of the ubiquitin-dependent proteolytic pathway. The murine homolog (Ufd1l) is expressed in the eyes and in the inner ear primordia during embryogenesis. In rat liver cytosol, Ufd1l binds to the nuclear transport protein Npl4 and this binary complex competes with p47 for binding to p97, which  inhibits Golgi membrane fusion. The tertiary complex Ufd1l/Npl4/p97 may also be involved in mitotic ubiquitin-dependent processes. Both Ufd1l and Npl4 are found in the nucleus, while p97 is found in the cytoplasm and nucleus. In humans, Ufd1L is primarily expressed in adult heart, placenta, skeletal muscle, and pancreas, and fetal liver and kidney. Thus, Ufd1L is thought to be involved in cell functions that are critical for normal cell development in the cardiac and craniofacial regions.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (4)

  1. Meyer HH, Shorter JG, Seemann J, Pappin D, Warren G. A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. EMBO J. 2000; 19(10):2181-2192. (Biology). View Reference
  2. Nagahama M, Suzuki M, Hamada Y, et al. SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation. Mol Biol Cell. 2003; 14(1):262-273. (Biology: Western blot). View Reference
  3. Pizzuti A, Novelli G, Ratti A. UFD1L, a developmentally expressed ubiquitination gene, is deleted in CATCH 22 syndrome. Hum Mol Genet. 1997; 6(2):259-265. (Biology). View Reference
  4. Yamagishi H, Garg V, Matsuoka R, Thomas T, Srivastava D. A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects. Science. 1999; 283(5405):1158-1161. (Biology). View Reference
View All (4) View Less
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.