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Purified Mouse Anti-Synapsin IIa
Purified Mouse Anti-Synapsin IIa

Western blot analysis of Synapsin IIa on a rat whole brain lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-synapsin IIa antibody.

Purified Mouse Anti-Synapsin IIa

Immunohistochemical staining with the mouse anti-synapsin IIa antibody on a rat brain section (formalin-fixed paraffin-embedded section with citrate buffer pretreatment; 40x magnification).

Western blot analysis of Synapsin IIa on a rat whole brain lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-synapsin IIa antibody.

Immunohistochemical staining with the mouse anti-synapsin IIa antibody on a rat brain section (formalin-fixed paraffin-embedded section with citrate buffer pretreatment; 40x magnification).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human (Tested in Development)
Mouse IgG1
Rat Synapsin IIa aa. 451-586
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-zinc-fixed, Immunoprecipitation (Tested During Development)
74 kDa
250 µg/ml
AB_397993
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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1/Synapsin IIa

Synapsins are a group of four related proteins (synapsin Ia, Ib, IIa, and IIb) found exclusively in neuronal tissues. The four identified synapsins originate by alternative splicing of the mRNA from two different genes (synapsin I and II). Phosphorylation of synapsins occurs concomitantly with membrane excitation and neurotransmitter release into the synaptic cleft. Synapsins bind to the synaptic vesicles through their amino terminal domain, which also contains phosphorylation sites for the cAMP-dependent protein kinase. In vitro, synapsins bundle actin filaments. This indicates that synapsins may cross-link the vesicles with the actin filaments at the presynaptic terminal. By using hippocampal neurons and specific anti-sense oligonucleotides to block synapsin IIa expression, it was found that synapsin IIa was essential for the formation of synapses.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (4)

  1. Ferreira A, Kosik KS, Greengard P, Han HQ. Aberrant neurites and synaptic vesicle protein deficiency in synapsin II-depleted neurons. Science. 1994; 264(5161):977-979. (Biology). View Reference
  2. Petersohn D, Schoch S, Brinkmann DR, Thiel G. The human synapsin II gene promoter. Possible role for the transcription factor zif268/egr-1, polyoma enhancer activator 3, and AP2. J Biol Chem. 1995; 270(41):24361-24369. (Biology). View Reference
  3. Sudhof TC, Czernik AJ, Kao HT, et al. Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins. Science. 1989; 245(4925):1474-1480. (Biology). View Reference
  4. Thiel G, Sudhof TC, Greengard P. Synapsin II. Mapping of a domain in the NH2-terminal region which binds to small synaptic vesicles. J Biol Chem. 1990; 265(27):16527-16533. (Biology). View Reference
View All (4) View Less
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.