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Purified Mouse Anti-RCC1
Purified Mouse Anti-RCC1

Western blot analysis of RCC1 on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RCC1 antibody.

Purified Mouse Anti-RCC1

Immunofluorescence staining of rabbit brain.

Western blot analysis of RCC1 on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RCC1 antibody.

Immunofluorescence staining of rabbit brain.

Product Details
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BD Transduction Laboratories™
Regulator of Chromosome Condensation-1
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human RCC1 aa. 19-176
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_397760
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610377 Rev. 1
Antibody Details
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9/RCC1

RCC1 (Regulator of Chromosome Condensation) is a chromatin-associated protein implicated in a number of cellular processes such as nuclei formation, initiation/termination of transcription, pre-mRNA splicing/3'-end formation, mRNA export and DNA replication. Normally, RCC1 appears to play a structural role with chromatin. This nuclear protein can function as a guanine nucleotide exchange factor for the Ras-like GTPase, Ran/TC4. Loss of RCC1 expression is related to a structural transition of the chromosome and correlates with the inability of the cells to detect unreplicated DNA. RCC1 appears to be necessary for nucleocytoplasmic transport of precursors of spliceosomal small nuclear RNAs (snRNAs), intranuclear transport of U3 snRNA and processing of ribosomal RNAs, but not for export of transfer RNAs.

610377 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610377 Rev.1
Citations & References
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Development References (5)

  1. Cheng Y, Dahlberg JE, Lund E. Diverse effects of the guanine nucleotide exchange factor RCC1 on RNA transport. Science. 1995; 267(5205):1807-1810. (Biology). View Reference
  2. Ciarallo S, Subramaniam V, Hung W. Altered p27(Kip1) phosphorylation, localization, and function in human epithelial cells resistant to transforming growth factor beta-mediated G(1) arrest. Mol Cell Biol. 2002; 22(9):2993-3002. (Biology: Western blot). View Reference
  3. Iborra FJ, Jackson DA, Cook PR. The path of RNA through nuclear pores: apparent entry from the sides into specialized pores. J Cell Sci. 2000; 113(2):291-302. (Biology: Electron microscopy). View Reference
  4. Ohtsubo M, Kai R, Furuno N. Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation. Genes Dev. 1987; 1(6):585-593. (Biology). View Reference
  5. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence). View Reference
View All (5) View Less
610377 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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