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Purified Mouse Anti-RanBP1
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human RanBP1 aa. 28-163
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
29 kDa
250 µg/ml
AB_398079
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610756 Rev. 1
Antibody Details
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35/RanBP1

Ran is a highly conserved GTPase that is ubiquitously expressed. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis, and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 29kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1. In addition, RanBP1 appears to regulate the activity of Ran-GAP1 (Ran-GTPase activating protein) by increasing GTP hydrolysis. RanBP1 also inhibits the function of the guanine nucleotide exchange factor RCC1 (regulator of chromosome condensation). Therefore, RanBP1 is responsible for the GTP-bound state of Ran and the increased GTP hydrolysis of Ran GTP.

610756 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610756 Rev.1
Citations & References
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Development References (3)

  1. Bischoff FR, Krebber H, Smirnova E, Dong W, Ponstingl H. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J. 1995; 14(4):705-715. (Biology). View Reference
  2. Chi NC, Adam EJ, Visser GD, Adam SA. RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import. J Cell Biol. 1996; 135(3):559-569. (Biology). View Reference
  3. Hayashi N, Yokoyama N, Seki T, Azuma Y, Ohba T, Nishimoto T. RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4. Mol Gen Genet. 1995; 247(6):661-669. (Biology). View Reference
610756 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.