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Purified Mouse Anti-Ninjurin
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG2a
Human Ninjurin aa. 1-152
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
18-22 kDa
250 µg/ml
AB_398098
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610776 Rev. 1
Antibody Details
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50/Ninjurin

Ninjurin is a protein whose expression is dramatically increased after sciatic nerve transection and crush injuries. The Ninjurin gene encodes for a protein of 152 amino acids with two putative transmembrane domains. Ninjurin was iodinated in vivo and promoted the aggregation of Jurkat cells expressing Ninjurin, indicating a portion of Ninjurin is exposed to the cell surface. The adhesive properties of Ninjurin were energy and temperature dependent, required Ca2+ and Mg2+, and the integrity of the cytoskeleton. Also, the adhesion domain of Ninjurin is located at the extracellular NH2-terminal domain. Although its predicted mass is 16 kDa, Ninjurin migrates as a 18-22 kDa protein in SDS-PAGE, depending on the cell line or tissue. mRNA analysis revealed that Ninjurin is widely expressed with the highest levels in liver, thymus, heart, and the lowest level in brain.

610776 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610776 Rev.1
Citations & References
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Development References (3)

  1. Araki T, Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron. 1996; 17(2):353-361. (Biology). View Reference
  2. Araki T, Zimonjic DB, Popescu NC, Milbrandt J. Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule. J Biol Chem. 1997; 272(34):21373-21380. (Biology). View Reference
  3. Chen JS, Coustan-Smith E, Suzuki T. Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia. Blood. 2001; 97(7):2115. (Clone-specific: Flow cytometry). View Reference
610776 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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