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Purified Mouse Anti-MCC
Purified Mouse Anti-MCC

Western blot analysis of MCC on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- MCC antibody.

Purified Mouse Anti-MCC

Immunofluorescence staining of human endothelial cells.

Western blot analysis of MCC on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- MCC antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Mutated in Colorectal Cancer
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human MCC aa. 5-146
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunoprecipitation (Not Recommended)
100 kDa
250 µg/ml
AB_398063
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610740 Rev. 1
Antibody Details
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1/MCC

The Mutated in Colorectal Cancer gene (MCC) was identified as a tumor suppressor that is deleted in sporadic and familial colorectal cancers. MCC is located at chromosome region 5q21. This region is often deleted in and associated with adenomas and carcinomas. Allele losses in the regions contiguous to MCC correlate with a high rate of colorectal tumors. MCC has been reported to be widely expressed and most abundant in epithelial cells and brain. It is a protein of 829 amino acids with a short sequence homologous to the m3 muscarinic acetylcholine receptor. Immunolocalization studies show MCC in the lateral plasma membrane of intestinal epithelial cells. In brain, MCC is abundant in the molecular layer of the cerebellum and is associated with various organelles, such as the plasma membrane.  Although overexpression of wild type MCC protein blocks progression through the cell cycle, a mutated MCC, that contains a Gln instead of Arg-506, does not. Therefore, wild type MCC functions as a  negative regulator of the cell cycle. Mutations or deletions in MCC might interfere with its physiological role and allow unrestricted cell proliferation (i.e. tumor formation).

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610740 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610740 Rev.1
Citations & References
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Development References (5)

  1. Ashton-Rickardt PG, Wyllie AH, Bird CC, et al. MCC, a candidate familial polyposis gene in 5q.21, shows frequent allele loss in colorectal and lung cancer. Oncogene. 1991; 6(10):1881-1886. (Biology). View Reference
  2. Huang JS, Chiang CP, Kok SH, Kuo YS, Kuo MY. Loss of heterozygosity of APC and MCC genes in oral squamous cell carcinomas in Taiwan. J Oral Pathol Med. 1997; 26(7):322-326. (Biology). View Reference
  3. Kinzler KW, Nilbert MC, Vogelstein B, et al. Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers. Science. 1991; 251(4999):1366-1370. (Biology). View Reference
  4. Matsumine A, Senda T, Baeg GH, et al. MCC, a cytoplasmic protein that blocks cell cycle progression from the G0/G1 to S phase. J Biol Chem. 1996; 271(17):10341-10346. (Biology). View Reference
  5. McKie AB, Filipe MI, Lemoine NR. Abnormalities affecting the APC and MCC tumour suppressor gene loci on chromosome 5q occur frequently in gastric cancer but not in pancreatic cancer. Int J Cancer. 1993; 55(4):598-603. (Biology). View Reference
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610740 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.