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Purified Mouse Anti-Human SMRT
Purified Mouse Anti-Human SMRT

Western blot analysis of SMRT on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-SMRT antibody.

Purified Mouse Anti-Human SMRT

Immunofluorescent staining of Wi38 cells with anti-SMRT antibody.

Western blot analysis of SMRT on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-SMRT antibody.

Immunofluorescent staining of Wi38 cells with anti-SMRT antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human SMRT aa. 1366-1473
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
340 kDa
250 µg/ml
AB_398909
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611387 Rev. 1
Antibody Details
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44/SMRT

Steroid nuclear hormone receptors regulate physiological homeostasis through repression and activation of gene transcription. In the absence of hormone, DNA-bound steroid receptors recruit corepressor, such as silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptorcorepressor (N-CoR), which bind to the free ligand binding domain of the thyroid hormone receptor, retinoic acid receptor, and other nuclear receptors. SMRT and N-CoR form a large protein complex with histone deacetylase I (HDAC1) and Sin3A, which deacetylates histones to alter chromatin structure in a manner that inhibits transcription. In addition, SMRT and N-CoR proteins interact with the transcription factor CBF/RBP-Jk to regulate Notch signaling pathways. SMRT contains four domains related to repressor activity (RD1, RD2, SRD1, and SRD2), two receptor interaction domains (RID1 and RID2), and a SANT region that is found in yeast chromatin remodeling factor SWI3. The ubiquitous expression pattern and corepressor activity of SMRT indicates that this protein is important for hormonal control of gene transcription in many tissues.

611387 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611387 Rev.1
Citations & References
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Development References (4)

  1. Chen JD, Evans RM. A transcriptional co-repressor that interacts with nuclear hormone receptors. Nature. 1995; 377(6548):454-457. (Biology). View Reference
  2. Nagy L, Kao HY, Chakravarti D, et al. Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase. Cell. 1997; 89(3):373-380. (Biology). View Reference
  3. Ordentlich P, Downes M, Xie W, Genin A, Spinner NB, Evans RM. Unique forms of human and mouse nuclear receptor corepressor SMRT. Proc Natl Acad Sci U S A. 1999; 96(6):2639-2644. (Biology). View Reference
  4. Wagner BL, Norris JD, Knotts TA, Weigel NL, McDonnell DP. The nuclear corepressors NCoR and SMRT are key regulators of both ligand- and 8-bromo-cyclic AMP-dependent transcriptional activity of the human progesterone receptor. Mol Cell Biol. 1998; 18(3):1369-1378. (Biology). View Reference
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611387 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.