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Purified Mouse Anti-Human SIP1
Purified Mouse Anti-Human SIP1

Western blot analysis of SIP1 on a K562 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human SIP1 antibody.

Purified Mouse Anti-Human SIP1

Immunofluorescence staining of human intestinal smooth muscle (HISM) cells.

Western blot analysis of SIP1 on a K562 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human SIP1 antibody.

Immunofluorescence staining of human intestinal smooth muscle (HISM) cells.

Product Details
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BD Transduction Laboratories™
SMN-Interacting Protein 1
Human (QC Testing)
Mouse IgG1
Human SIP1 aa. 36-156
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
32 kDa
250 µg/ml
AB_398786
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611256 Rev. 1
Antibody Details
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4/SIP1

Spinal muscular atrophy (SMA), a commonly fatal autosomal recessive disease, is caused by the degeneration of spinal anterior horn cells. It leads to symmetrical limb and trunk paralysis and muscular atrophy. SMA has been linked to the protein product of the Survival of Motor Neurons (SMN) gene. In greater than 98% of all SMA patients, SMN has been reported to be deleted or mutated. Although its function is unknown, the SMN protein is highly concentrated in novel nuclear structures, termed gems. SIP1 (SMN-interacting protein 1) forms a stable heteromeric complex with SMN and colocalizes with SMN in gems and in the cytoplasm.  In SMA, the expression of both proteins is dramatically reduced in motor neurons. Additionally, SMN and SIP1 have been isolated from a 300 kDa protein complex that also contains spliceosomal snRNP proteins. In particular, the SMN-SIP1 complex associates with the spliceosomal snRNAs U1 and U5. Antibodies against the SMN-SIP1 complex interfere with the assembly and nuclear importation of spliceosomal complexes. Thus, it is thought that the SMN-SIP1 complex mediates the formation of spliceosomal snRNPs. However, the exact role of SIP1 in rRNA processing has yet to be determined.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611256 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611256 Rev.1
Citations & References
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Development References (2)

  1. Fischer U, Liu Q, Dreyfuss G. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis. Cell. 1997; 90(6):1023-1029. (Biology). View Reference
  2. Liu Q, Fischer U, Wang F, Dreyfuss G. The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell. 1997; 90(6):1013-1021. (Biology). View Reference
611256 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.