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Purified Mouse Anti- Human MCAM
Purified Mouse Anti- Human MCAM

Western blot analysis of MCAM on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- MCAM antibody.

Purified Mouse Anti- Human MCAM

Immunofluorescent staining of human endothelial cells.

Western blot analysis of MCAM on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- MCAM antibody.

Immunofluorescent staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human MCAM aa. 104-305
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
113 kDa
250 µg/ml
AB_398742
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611208 Rev. 1
Antibody Details
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1/MCAM

Tumor metastasis is determined by the metastatic capacity of subpopulations of tumor cells. The presence of cell adhesion molecules (CAMs) is a property of tumor cells that is essential for metastasis. MCAM (MUC18 or Mel-CAM) was identified on melanoma cells and is one of several CAMs that belong to the immunoglobulin superfamily. MCAM is an integral membrane glycoprotein that consists of five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic domain. The presence of several protein kinase recognition motifs in the cytoplasmic domain suggest a role for MCAM in signal transduction. MCAM is capable of both homotypic and heterotypic adhesion between cells, but its heterotypic ligand remains to be identified. It is sparsely expressed on benign melanocytic nevi and thin primary melanomas. However, MCAM expression increases with tumor thickness. It is expressed on 70-80% of advanced primary and metastatic tumors. Additionally, S-Endo-1 antigen (CD146), present on human endothelial cells, is identical to MCAM. Thus, MCAM may play an important role in the development of metastatic tumors and possibly represents a prime target for therapeutic treatment.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611208 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611208 Rev.1
Citations & References
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Development References (4)

  1. Anfosso F, Bardin N, Frances V, et al. Activation of human endothelial cells via S-endo-1 antigen (CD146) stimulates the tyrosine phosphorylation of focal adhesion kinase p125(FAK). J Biol Chem. 1998; 273(41):26852-26856. (Biology). View Reference
  2. Jean D, Gershenwald JE, Huang S, et al. Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor growth and metastasis of human melanoma cells. J Biol Chem. 1998; 273(26):16501-16508. (Biology). View Reference
  3. Lehmann JM, Riethmuller G, Johnson JP. MUC18, a marker of tumor progression in human melanoma, shows sequence similarity to the neural cell adhesion molecules of the immunoglobulin superfamily. Proc Natl Acad Sci U S A. 1989; 86(24):9891-9895. (Biology). View Reference
  4. Xie S, Luca M, Huang S, et al. Expression of MCAM/MUC18 by human melanoma cells leads to increased tumor growth and metastasis. Cancer Res. 1997; 57(11):2295-2303. (Biology). View Reference
View All (4) View Less
611208 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.