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Purified Mouse Anti-hHR23B
Purified Mouse Anti-hHR23B

Western blot analysis of hHR23B on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-hHR23B antibody.

Purified Mouse Anti-hHR23B

Immunofluorescence staining of WiDr cells (Human colorectal adenocarcinoma; ATCC CCL-218).

Western blot analysis of hHR23B on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-hHR23B antibody.

Immunofluorescence staining of WiDr cells (Human colorectal adenocarcinoma; ATCC CCL-218).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human hHR23B aa. 73-193
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
58 kDa
250 µg/ml
AB_398332
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611018 Rev. 1
Antibody Details
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16/hHR23B

Nucleotide excision repair (NER) is an essential element of DNA repair which eliminates unrelated DNA base lesions, including those induced by UV irradiation or chemicals.  Although well defined in prokaryotes, eukaryotic NER is complex and involves at least two distinct subpathways.  The transcription-coupled repair subpathway eliminates DNA lesions just prior to transcription.  This is important for the removal of lesions for which the second pathway, global genome repair (i.e. covers the entire genome), is unable to repair.  Individuals with defects in both pathways may suffer from xeroderma pigmentosum (XP), a condition with increased incidence of cancer in skin and internal organs.  Mutations in seven genes result in human XP. However, one of these, XP-C, affects only the global genome repair pathway.  The XP-C protein interacts with hHR23B, the human homolog of RAD23, an essential component of yeast NER.  The XPC-hHR23B complex is required for excision of thymine dimers.  In addition, hHR23B contains an N-terminal ubiquitin-like domain that allows for proteasome interaction.  Thus, hHR23B may be an essential component of NER and mechanisms of DNA repair.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.

611018 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611018 Rev.1
Citations & References
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Development References (5)

  1. Masutani C, Sugasawa K, Yanagisawa J. Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23. EMBO J. 1994; 13(8):1831-1843. (Biology). View Reference
  2. Reardon JT, Mu D, Sancar A. Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease. J Biol Chem. 1996; 16(32):4852-4861. (Biology). View Reference
  3. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence). View Reference
  4. Sugasawa K, Masutani C, Uchida A. HHR23B, a human Rad23 homolog, stimulates XPC protein in nucleotide excision repair in vitro. Mol Cell Biol. 1996; 16(9):4852-4861. (Biology). View Reference
  5. Wakasugi M, Sancar A. Assembly, subunit composition, and footprint of human DNA repair excision nuclease. Proc Natl Acad Sci U S A. 1998; 95(12):6669-6674. (Biology). View Reference
View All (5) View Less
611018 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.