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Purified Mouse Anti-FKBP51
Purified Mouse Anti-FKBP51

Western blot analysis using the Mouse Anti-FKBP51 antibody on a human endothelial cell lysate.

Lane 1: 1 µg/mL, lane 2: 0.5 µg/mL, lane 3: 0.25 µg/mL of the Mouse Anti-FKBP51 antibody was used.

Purified Mouse Anti-FKBP51

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis using the Mouse Anti-FKBP51 antibody on a human endothelial cell lysate.

Lane 1: 1 µg/mL, lane 2: 0.5 µg/mL, lane 3: 0.25 µg/mL of the Mouse Anti-FKBP51 antibody was used.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
Mouse IgG1
Mouse FKBP51 aa. 4-199
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
51-55 kDa
250 µg/ml
AB_2246893
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610582 Rev. 2
Antibody Details
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18/FKBP51

FK506, one of several potent compounds that block T cell proliferation, is used in immunosuppressive therapy. Several FK506-binding proteins (FKBPs) have been identified in the search for intracellular targets of FK506. These proteins include FKBP12, FKBP12.6, and FKBP52. The immunosuppressive effects of FK506  and FKBPs result from the inhibition of the calcineurin phosphatase, a well known component of the signaling cascade leading to IL-2 production. Calcineurin and FKBP's are widely distributed, therefore, it is difficult to achieve immunosuppressive specificity by FK506. FKBP51 (51kDa) is an FK506-binding protein that is expressed exclusively in T cells and has homology to FKBP52. Because of its limited distribution, FKBP51 may provide a more selective target for FK506 in T cells.

610582 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610582 Rev.2
Citations & References
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Development References (1)

  1. Baughman G, Wiederrecht GJ, Campbell NF, Martin MM, Bourgeois S. FKBP51, a novel T-cell-specific immunophilin capable of calcineurin inhibition. Mol Cell Biol. 1995; 15(8):4395-4402. (Biology). View Reference
610582 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.