Skip to main content Skip to navigation
Purified Mouse Anti-ERK1
Purified Mouse Anti-ERK1

Western blot analysis of ERK1 on a rat cerebrum lysate. 1:4000 (lane 1), 1:8000 (lane 2), 1:16,000 (lane 3) dilution of the mouse anti-ERK1 antibody. ERK1 is expected to appear at 44 kD with crossreactivity reported to ERK2 which may appear at 42 kD.

Purified Mouse Anti-ERK1

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of ERK1 on a rat cerebrum lysate. 1:4000 (lane 1), 1:8000 (lane 2), 1:16,000 (lane 3) dilution of the mouse anti-ERK1 antibody. ERK1 is expected to appear at 44 kD with crossreactivity reported to ERK2 which may appear at 42 kD.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Rat ERK1 aa. 325-345
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
44 kDa
250 µg/ml
AB_397790
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610408 Rev. 1
Antibody Details
Down Arrow Up Arrow
MK1

The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton.  Activation of ERK is normally transient and cells possess dual specificity phosphotases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kD).

610408 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610408 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (4)

  1. Boulton TG, Cobb MH. Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies. Cell Regul. 1991; 2(5):357-371. (Biology). View Reference
  2. Clark EA, Hynes RO. Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization. J Biol Chem. 1996; 271(25):14814-14818. (Biology). View Reference
  3. Cobb MH, Goldsmith EJ. How MAP kinases are regulated. J Biol Chem. 1995; 270(25):14843-14846. (Biology). View Reference
  4. Radoja S, Saio M, Schaer D, Koneru M, Vukmanovic S, Frey AB. CD8(+) tumor-infiltrating T cells are deficient in perforin-mediated cytolytic activity due to defective microtubule-organizing center mobilization and lytic granule exocytosis. J Immunol. 2001; 167(9):5042-5051. (Biology: Western blot). View Reference
View All (4) View Less
610408 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.