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Purified Mouse Anti-AMPK β
Product Details
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BD Transduction Laboratories™
AMP activated protein kinase
Mouse (QC Testing), Human, Rat, Dog, Frog (Tested in Development)
Mouse IgG1
Rat AMPK β aa. 109-247
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
38 kDa
250 µg/ml
AB_398121
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610802 Rev. 1
Antibody Details
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12/AMPKβ

AMP activated protein kinase (AMPK) was identified as a result of its phosphorylation and inactivation of lipid metabolism enzymes.  However, AMPK also phosphorylates enzymes involved in other metabolic pathways.  AMPK is activated by AMPKK (AMPK Kinase)-mediated phosphorylation in response to intracellular changes in AMP levels.  Although rat skeletal muscle AMPK exists as a monomer, active rat liver AMPK is a heterotrimer of α (catalytic subunit), β, and γ subunits.  The catalytic subunit (63 kDa) is structurally and functionally related to the S. cerevisiae SNF1 protein kinase, an enzyme that is required for expression of glucose repressed genes during glucose starvation.  AMPK β (38 kDa) is strongly related to SIP2 (35% identity) and contains a C-terminal region that is very homologous to the SIP1/SIP2/GAL83 ASC domain.  AMPK β has some identity (35%) with SNF4, which is necessary for SNF1 activation.  Both AMPK β and γ are widely expressed in rat tissues.  AMPK β facilitates the interactions of the α and γ subunits in vitro.  Although AMPK β is clearly necessary for formation of the heterotrimeric complex, there are other probably, yet unknown, roles for this protein.

610802 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610802 Rev.1
Citations & References
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Development References (4)

  1. Michell BJ, Stapleton D, Mitchelhill KI, et al. Isoform-specific purification and substrate specificity of the 5'-AMP-activated protein kinase. J Biol Chem. 1996; 271(45):28445-28450. (Biology). View Reference
  2. Mitchelhill KI, Michell BJ, House CM, . Posttranslational modifications of the 5'-AMP-activated protein kinase beta1 subunit. J Biol Chem. 1997; 272(39):24475-24479. (Biology). View Reference
  3. Winder WW, Wilson HA, Hardie DG, et al. Phosphorylation of rat muscle acetyl-CoA carboxylase by AMP-activated protein kinase and protein kinase A.. J Appl Physiol. 1997; 82(1):219-25. (Biology). View Reference
  4. Woods A, Cheung PC, Smith FC, et al. Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro. J Biol Chem. 1996; 271(17):10282-10290. (Biology). View Reference
View All (4) View Less
610802 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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