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Purified Mouse Anti-AKAP-KL
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Rat (Tested in Development)
Mouse IgG1
Mouse AKAP-KL aa. 627-746
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
105-130 kDa
250 µg/ml
AB_398445
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611134 Rev. 1
Antibody Details
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41/AKAP-KL

Cyclic AMP (cAMP) is a common second messenger for a variety of cytokines, hormones, and neurotransmitters. Central to this signaling pathway is the activation of cAMP-dependent protein kinase A (PKA). The non-uniform intracellular distribution of PKA isoforms allows for the delivery of cAMP-transmitted signals to discrete subcellular sites. This is mediated by the attachment of PKA isoforms to the cytoskeleton or organelles by AKAPs. AKAPs simultaneously bind to the RII subunits of PKAII and to the cytoskeleton. At least six AKAP-KL (A kinase anchor proteins expressed in lung and kidney) proteins are generated by alternative mRNA splicing. They are present primarily in lung, kidney, and cerebellum and are absent from many other tissues. All isoforms consists of a 20-residue RII interaction domain. Additionally, AKAP-KL binds and regulates the actin cytoskeleton and exhibits a polarized distribution in situ. Thus, AKAP-KL plays a role in establishing polarity in signaling systems and also functions to focus and amplify cAMP-mediated signals by integrating PKA isoforms with downstream effector molecules.

611134 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611134 Rev.1
Citations & References
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Development References (1)

  1. Dong F, Feldmesser M, Casadevall A, Rubin CS. Molecular characterization of a cDNA that encodes six isoforms of a novel murine A kinase anchor protein. J Biol Chem. 1998; 273(11):6533-6541. (Biology). View Reference
611134 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.