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FITC Mouse Anti-p21-Arc
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human p21-Arc aa. 10-118
Immunofluorescence (Routinely Tested)
250 µg/ml
AB_399559
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

For use in Western blotting, the unconjugated, purified format,  Cat. No. 612234 is recommended

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612236 Rev. 1
Antibody Details
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26/p21-Arc

Cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments occurs through the interactions between actin and actin binding proteins.  Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles.  Actin related proteins (Arp) 2/3 complex is an actin polymerization inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc.  The Arp2 and Arp3 subunits may nucleate actin polymerization, while the p41-Arc subunit is a WD repeat-containing protein that may regulate both the activity and localization of the Arp2/3 complex.  Arp3, p34-Arc, and p21-Arc are localized to the lamellipodia of stationary and locomoting fibroblasts.  Both WASP and Abp1p are acidic sequence-containing proteins that activate the Arp2/3 complex.  However, WASP binds actin monomers, while the endocytosis-related Abp1p protein binds actin filaments.  Thus, Arp2/3 complex may regulate actin polymerization in specific cell locations through interaction with actin binding Arp2/3 activators.

612236 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
612236 Rev.1
Citations & References
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Development References (2)

  1. Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997; 138(2):375-384. (Biology). View Reference
  2. Zhao X, Yang Z, Qian M, Zhu X. Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochem Biophys Res Commun. 2001; 280(2):513-517. (Biology). View Reference
612236 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.