HRP Rat Anti-Mouse Ig, κ Light Chain

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BD Pharmingen™ HRP Rat Anti-Mouse Ig, κ Light Chain

Name: HRP Rat Anti-Mouse Ig, κ Light Chain
Brand: BD Pharmingen™
SKU: 559751

Clone 187.1

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Ig kappa chain C region; IGKC; Igk-C; Ig, κ

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ

Immunogen: Mouse IgG2b κ secreted by MPC-11 plasmacytoma

Application: ELISA (Routinely Tested)

Entrez Gene ID: 16071

RRID: AB_397315

Storage Buffer: Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

Recommended Assay Procedures

Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000.

Warning: HRP Rat Anti-Mouse Ig, k Light Chain contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
ProClin is a trademark of Rohm and Haas Company.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

559751 Rev. 9

Antibody Details

187.1

The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.

559751 Rev. 9

Format Details

HRP

Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.

Format: HRP

559751 Rev.9

Citations & References

Development References (1)

Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference

559751 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.