ELISA Detection: The biotinylated MP2-43D11 antibody (Cat. No. 554384) is useful as a detection antibody for a sandwich ELISA for measuring mouse IL-3 protein levels. Biotinylated MP2-43D11 antibody can be paired with the purified MP2-8F8 antibody (Cat. No. 554381) as the capture antibody, with recombinant mouse IL-3 protein (Cat. No. 554579) as the standard. Biotinylated MP2-43D11 antibody should be titrated 0.5 - 2.0 µg/ml to determine optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of mouse IL-3 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
Note: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For testing mouse IL-3 in serum or plasma, the BD OptEIA™ ELISA Set (Cat. No. 555228) is recommended.
Western Blot: The MP2-43D11 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.