The AK-7 monoclonal antibody specifically binds to CD49b. CD49b is also known as integrin α2 chain, VLA-α2, and GP1a. CD49b is a type I transmembrane glycoprotein and belongs to the integrin family of extracellular matrix and cell-cell adhesion receptors. CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b noncovalently associates with CD29 to form the VLA-2 complex (integrin α2/β1). The VLA-2 complex serves as a receptor for collagen and laminin. It can regulate responses mediated by proinflammatory effector cells.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.