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Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Companion Products
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The 11G2 monoclonal antibody specifically binds to CD156c which is also known as Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). CD156c is a ~70 kDa type I transmembrane glycoprotein that belongs to the ADAM family. It is widely expressed on hematopoietic and non-hematopoietic cells. CD156c is expressed on the plasma membrane, as well as by membranes within intracellular compartments and exosomes. CD156c serves as a broadly-reactive endopeptidase that cleaves membrane-bound proteins in a process known as ectodomain shedding or release. It can cleave transmembrane molecules into soluble forms and thereby regulate the functions of a variety of different receptors and ligands that are involved in cellular signaling and adhesion. These include membrane TNF, CX3CL1, CXCL16, Ephrin-A2, Notch and Delta molecules, cadherins, CD23, CD44, IL-6 Receptor/CD126, CD171, and amyloid precursor protein. CD156c expression may be constitutive or induced, such as in inflamed tissues, including arthritic joints and in the nervous system. Dysregulated CD156c expression may be associated with certain cancers and Alzheimer's disease.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.
Development References (7)
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Arduise C, Abache T, Li L, et al. Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor.. J Immunol. 2008; 181(10):7002-13. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunoaffinity chromatography, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Huovila AP, Turner AJ, Pelto-Huikko M, Kärkkäinen I, Ortiz RM. Shedding light on ADAM metalloproteinases.. Trends Biochem Sci. 2005; 30(7):413-22. (Biology). View Reference
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Pruessmeyer J, Ludwig A. The good, the bad and the ugly substrates for ADAM10 and ADAM17 in brain pathology, inflammation and cancer.. Semin Cell Dev Biol. 2009; 20(2):164-74. (Biology). View Reference
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Saftig P, Lichtenthaler SF. The alpha secretase ADAM10: A metalloprotease with multiple functions in the brain.. Prog Neurobiol. 2015; 135:1-20. (Biology). View Reference
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Stoeck A, Keller S, Riedle S, et al. A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44.. Biochem J. 2006; 393(Pt 3):609-18. (Clone-specific: Flow cytometry, Immunoprecipitation, Western blot). View Reference
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Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference
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Zola H, Swart B, Nicholson I, Voss E. CD156c. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:288.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
