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Purified Mouse Anti-Rat CD106
Product Details
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BD Pharmingen™
Vcam1; V-CAM 1; VCAM-1; VCAM1B; vascular cell adhesion protein 1
Rat (QC Testing)
Mouse IgG1, κ
Rat VCAM-1-transfected L5178Y
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559165 Rev. 5
Antibody Details
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The MR106 monoclonal antibody specifically recognizes CD106, the ~110 kDa transmembrane vascular cell adhesion molecule-1 (VCAM-1). Expression of CD106 on endothelial cells is induced by inflammatory cytokines and in certain pathologic conditions. In the rat bone marrow, CD106 is reportedly expressed on dendritic cells and subsets of the myeloid lineage, but not detectable on peripheral leucocytes. CD106 can reportedly serve as a counter-receptor for VLA-4 (α4β1 integrin) and α4β7 integrin, and it plays an important role in leucocyte extravasation, particularly at sites of inflammation.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

559165 Rev. 5
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
559165 Rev.5
Citations & References
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View product citations for antibody "559165" on CiteAb

Development References (1)

  1. Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol. 1993; 11:767-804. (Biology). View Reference
559165 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.