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      Purified Mouse Anti-Human Myeloperoxidase
      Product Details
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      BD Pharmingen™
      MPO
      Human (QC Testing)
      Mouse IgG1, κ
      Immunohistochemistry-zinc-fixed (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Intracellular staining (flow cytometry) (Tested During Development)
      0.5 mg/ml
      AB_396309
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      556035 Rev. 7
      Antibody Details
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      1B10

      Reacts with myeloperoxidase (MPO), a hemoprotein of 140 kDa, composed of two heavy subunits of 52 kDa and two light chains of 15 kDa. MPO is stored in primary azurophilic granules of neutrophils and plays a major role in the bactericidal activity of neutrophils during phagocytosis. It catalyzes the generation of hypochlorous acid, a powerful oxidant. 1B10 antibody detects MPO in neutrophils, monocytes and HL-60 cells. This reagent is suitable for immunohistochemical staining of acetone-fixed, frozen tissue section, or formalin-fixed, paraffin-embedded tissue sections with TUF pretreatment, or zinc-fixed, paraffin-embedded tissue sections with no pretreatment.

      556035 Rev. 7
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      556035 Rev.7
      Citations & References
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      Development References (2)

      1. Audrain MA, Baranger TA, Moguilevski N, et al. Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies. Clin Exp Immunol. 1997; 107(1):127-134. (Biology). View Reference
      2. Lanza F, Latorraca A, Moretti S, Castagnari B, Ferrari L, Castoldi G. Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells. Cytometry. 1997; 30(3):134-144. (Biology). View Reference
      556035 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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