Flow cytometric analysis of CD221 expression on Jurkat cells. Jurkat cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD221 (Cat. No. 556000; solid line histogram). CD221 and Ig isotype expression were visualized with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of CD221 expression on Jurkat cells. Jurkat cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD221 (Cat. No. 556000; solid line histogram). CD221 and Ig isotype expression were visualized with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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The 3B7 monoclonal antibody specifically recognizes CD221which is also known as the human Insulin-like Growth Factor-I Receptor (IGF-I Receptor, IGFIR, or IGF1R). CD221 is the receptor for insulin-like growth factors IGF-I and IGF-II. CD221 is expressed as a glycoprotein complex composed of two extracellular α subunits that are disulfide linked to two transmembrane β subunits that have tyrosine kinase domains. The transmembrane subunits play a role in tyrosine phosphorylation of several intracellular signaling proteins. CD221 is structurally and functionally similar to CD220 (Insulin Receptor) and is expressed on a variety of human hematopoietic and non-hematopoietic cells. The 3B7 antibody does not crossreact with CD220. The 3B7 antibody stimulates IGF-I and IGF-II induced cell growth and activates autophosphorylation of the CD221 β subunit.
Development References (5)
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Kooijman RK, Scholtens LE, Rijkers GT, Zegers BJ. Differential expression of type I insulin-like growth factor receptors in different stages of human T cells. Eur J Immunol. 1995; 25(4):931-935. (Biology). View Reference
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Li SL, Kato J, Paz IB, Kasuya J, Fujita-Yamaguchi Y. Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor. Biochem Biophys Res Commun. 1993; 196(1):92-98. (Immunogen). View Reference
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Siddle K. CD220 (insulin receptor) and CD221 (type I insulin-like growth factor receptor) Summary and Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:473-482.
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Xiong L, Kasuya J, Li SL, Kato J, Fujita-Yamaguchi Y. Growth-stimulatory monoclonal antibodies against human insulin-like growth factor I receptor. Proc Natl Acad Sci U S A. 1992; 89(12):5356-5360. (Biology). View Reference
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Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
