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Purified Mouse Anti-Human CD221
Purified Mouse Anti-Human CD221

Flow cytometric analysis of CD221 expression on Jurkat cells. Jurkat cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD221 (Cat. No. 556000; solid line histogram). CD221 and Ig isotype expression were visualized with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™.  

Flow cytometric analysis of CD221 expression on Jurkat cells. Jurkat cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram) or Purified Mouse Anti-Human CD221 (Cat. No. 556000; solid line histogram). CD221 and Ig isotype expression were visualized with Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™.  

Product Details
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BD Pharmingen™
IGF1R; IGF-I receptor; IGFIR; IGFR; JTK13
Human (QC Testing)
Mouse BALB/c IgG1, κ
Purified Human IGF-I Receptors
Flow cytometry (Routinely Tested), Immunofluorescence (Reported)
0.5 mg/ml
VII 70315
AB_396284
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556000 Rev. 8
Antibody Details
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3B7

The 3B7 monoclonal antibody specifically recognizes CD221which is also known as the human Insulin-like Growth Factor-I Receptor (IGF-I Receptor, IGFIR, or IGF1R). CD221 is the receptor for insulin-like growth factors IGF-I and IGF-II. CD221 is expressed as a glycoprotein complex composed of two extracellular α subunits that are disulfide linked to two transmembrane β subunits that have tyrosine kinase domains. The transmembrane subunits play a role in tyrosine phosphorylation of several intracellular signaling proteins. CD221 is structurally and functionally similar to CD220 (Insulin Receptor) and is expressed on a variety of human hematopoietic and non-hematopoietic cells. The 3B7 antibody does not crossreact with CD220. The 3B7 antibody stimulates IGF-I and IGF-II induced cell growth and activates autophosphorylation of the CD221 β subunit.

556000 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556000 Rev.8
Citations & References
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Development References (5)

  1. Kooijman RK, Scholtens LE, Rijkers GT, Zegers BJ. Differential expression of type I insulin-like growth factor receptors in different stages of human T cells. Eur J Immunol. 1995; 25(4):931-935. (Biology). View Reference
  2. Li SL, Kato J, Paz IB, Kasuya J, Fujita-Yamaguchi Y. Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor. Biochem Biophys Res Commun. 1993; 196(1):92-98. (Immunogen). View Reference
  3. Siddle K. CD220 (insulin receptor) and CD221 (type I insulin-like growth factor receptor) Summary and Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:473-482.
  4. Xiong L, Kasuya J, Li SL, Kato J, Fujita-Yamaguchi Y. Growth-stimulatory monoclonal antibodies against human insulin-like growth factor I receptor. Proc Natl Acad Sci U S A. 1992; 89(12):5356-5360. (Biology). View Reference
  5. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Biology). View Reference
View All (5) View Less
556000 Rev. 8

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.