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PE Rat Anti-Mouse CD195
PE Rat Anti-Mouse CD195

Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 µg/ml, final concentration; Sigma). The splenocytes were cultured for additional 48 hours in the presence of mouse IL-2 (100 U/ml, final concentration; Cat. No. 550069). The splenocytes were then stained with PE-conjugated rat anti- mouse CD195 antibody (C34-3448,) and PE-Cy5 conjugated hamster antimouse CD3 (145-2C11, Cat. No. 553065) following BD Pharmingen's staining protocol. The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the PE-conjugated rat IgG2c isotype control (PE-A23-1; Cat. No. 559841, data not shown.). The quadrant markers for the bivariate dot plots were set based on the isotype controls.

Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 µg/ml, final concentration; Sigma). The splenocytes were cultured for additional 48 hours in the presence of mouse IL-2 (100 U/ml, final concentration; Cat. No. 550069). The splenocytes were then stained with PE-conjugated rat anti- mouse CD195 antibody (C34-3448,) and PE-Cy5 conjugated hamster antimouse CD3 (145-2C11, Cat. No. 553065) following BD Pharmingen's staining protocol. The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the PE-conjugated rat IgG2c isotype control (PE-A23-1; Cat. No. 559841, data not shown.). The quadrant markers for the bivariate dot plots were set based on the isotype controls.

Product Details
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BD Pharmingen™
Ccr5; chemokine (C-C) receptor 5; C-C CKR-5; CC-CKR-5; CCR-5; AM4-7
Mouse (QC Testing)
Rat IgG2c, κ
Mouse CCR5 aa. 9-30
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397377
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometry Analysis: The PE-conjugated C34-3448 antibody can be used for multicolor immunofluorescent staining and flow cytometric analysis to identify and enumerate CD195-expressing cells within mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, the antibody should be titrated (≤  0.25 µg mAb/million cells). For specific methodology, please visit the onnline protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559923 Rev. 1
Antibody Details
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C34-3448

The C34-3448 monoclonal antibody specifically binds to CD195 which is also known as, C-C chemokine receptor type 5 (CCR5). CD195 is a seven transmembrane-spanning G-protein-coupled receptor that belongs to the β-chemokine receptor family. CD195 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals in response to at least three chemokines: CCL3/MIP-1α, CCL4/MIP-1β, and CCL5/RANTES. CD195 is expressed on macrophages and some T-lymphocytes.

559923 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559923 Rev.1
Citations & References
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Development References (3)

  1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). View Reference
  2. Meyer A, Coyle AJ, Proudfoot AE, Wells TN, Power CA. Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor. J Biol Chem. 1996; 271(24):14445-14451. (Biology). View Reference
  3. Napolitano M, Seamon KB, Leonard WJ. Identification of cell surface receptors for the Act-2 cytokine. J Exp Med. 1990; 172(1):285-289. (Biology). View Reference
559923 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.