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PE Rat Anti-Mouse CD141
PE Rat Anti-Mouse CD141

Two-color flow cytometric analysis of CD141 expressed on BALB/c mouse peritoneal exudate cells (PECs). PECs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 antibody (Cat. No. 565411) and either 0.06 μg of PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD141 antibody (Cat. No. 566338; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD141 (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

PE Rat Anti-Mouse CD141

Two-color flow cytometric analysis of CD141 expressed on BALB/c mouse bone marrow cells (BMCs). BMCs were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either 0.06 μg of PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD141 antibody (Cat. No. 566338; Right Plot). Two-color contour plots showing the correlated expression of CD141 (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

Two-color flow cytometric analysis of CD141 expressed on BALB/c mouse peritoneal exudate cells (PECs). PECs were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 antibody (Cat. No. 565411) and either 0.06 μg of PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD141 antibody (Cat. No. 566338; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD141 (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

Two-color flow cytometric analysis of CD141 expressed on BALB/c mouse bone marrow cells (BMCs). BMCs were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either 0.06 μg of PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD141 antibody (Cat. No. 566338; Right Plot). Two-color contour plots showing the correlated expression of CD141 (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
TM; Thbd; fetomodulin; thrombomodulin
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
Mouse LyEnd.5 Cell Membranes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739695
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566338 Rev. 2
Antibody Details
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LS17-9

The LS17-9 monoclonal antibody specifically binds to CD141, also known as thrombomodulin (TM or TRBM) or fetomodulin. CD141 is encoded by Thbd and expressed as a type I transmembrane glycoprotein with an N-terminal C-type lectin domain followed by EGF-like repeats. CD141 is expressed on a variety of cells including endothelial cells, epithelial cells, mesothelial cells, keratinocytes, synovial lining cells, fibroblastic reticular cells, megakaryocytes, dendritic cells, monocytes, macrophages, neutrophils, precursor B cells, and hematopoietic progenitor cells. CD141 is required for normal fetal development. CD141 binds thrombin and this complex can activate Protein C and Thrombin activatable fibrinolysis inhibitor (TAFI), leading to anticoagulant and antifibrinolytic pathway activities.

        

566338 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566338 Rev.2
Citations & References
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Development References (4)

  1. Conway EM, Pollefeyt S, Cornelissen J, et al. Structure-function analyses of thrombomodulin by gene-targeting in mice: the cytoplasmic domain is not required for normal fetal development.. Blood. 1999; 93(10):3442-50. (Biology). View Reference
  2. Frontera V1, Arcangeli ML, Zimmerli C, et al. Cutting edge: JAM-C controls homeostatic chemokine secretion in lymph node fibroblastic reticular cells expressing thrombomodulin.. J Immunol. 2011; 187(2):603-607. (Immunogen: Cell separation, Flow cytometry, Fluorescence activated cell sorting, Fluorescence microscopy, Immunofluorescence, Immunoprecipitation). View Reference
  3. Li YH, Kuo CH, Shi GY, Wu HL. The role of thrombomodulin lectin-like domain in inflammation.. J Biomed Sci. 2012; 19:34. (Biology). View Reference
  4. Mionnet C, Mondor I, Jorquera A, et al. Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.. PLoS Biol. 2013; 11(10):e1001672. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
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566338 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.