PE Mouse Anti-Mouse Qa-1(b)
Flow cytometric analysis of Qa-1[b] expression on lipopolysaccharide-activated C57BL/6 mouse splenocytes. Splenic leucocytes from an A/J (Qa-1[a]; Left Panel) or C57BL/6 (Qa-1[b]; Right Panel) mouse were stimulated for 3 days in culture with lipopolysaccharide (LPS). The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histograms) or PE Mouse Anti-Mouse Qa-1[b] antibody (Cat. No. 566640/566641; solid line histograms) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histograms showing Qa-1[b] expression (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative), activated splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Mouse Qa-1(b)
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