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PE Mouse Anti-Human NKp80
PE Mouse Anti-Human NKp80

Flow cytometric analysis of NKp80 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD56 antibody (Cat. No. 562751) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plot) or PE Mouse Anti-Human NKp80 antibody (Cat. No. 566329; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of NKp80 (or Ig Isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Flow cytometric analysis of NKp80 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD56 antibody (Cat. No. 562751) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plot) or PE Mouse Anti-Human NKp80 antibody (Cat. No. 566329; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of NKp80 (or Ig Isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Product Details
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BD Pharmingen™
KLRF1; CLEC5C; C-type lectin domain family 5 member C; ML
Human (QC Testing)
Mouse IgG1, κ
Human NKp80 extracellular domain
Flow cytometry (Routinely Tested)
5 µl/test
AB_2739689
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566329 Rev. 1
Antibody Details
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5D12

The 5D12 monoclonal antibody specifically binds to NKp80 which is also known as C-type lectin domain family 5 member C (CLEC5C). NKp80 is a single-pass type II transmembrane glycoprotein that contains a C-type lectin extracellular domain and is expressed as a ~80 kDa disulfide-linked homodimer. NKp80 is encoded by KLRF1 (Killer cell lectin-like receptor subfamily F member 1) which maps to the NK-gene complex (NKC). NKp80 is expressed on most NK cells, and subsets of γδ T cells and effector-memory CD8 αβ T cells. NKp80 is not present in rodents. NKp80 is an activating coreceptor that induces NK cell-mediated cytotoxicity and proinflammatory cytokine (eg, IFN-γ and TNF) production upon interaction with its ligand, AICL (Activation induced C type lectin). AICL is encoded by CLEC2B that is selectively expressed on myeloid cells including activated monocytes, macrophages, and granulocytes. NKp80 can reportedly transduce signals through an atypical hemi-ITAM-like sequence motif and Syk kinase to trigger cellular responses.

        

566329 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566329 Rev.1
Citations & References
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Development References (4)

  1. Bartel Y, Bauer B, Steinle A. Modulation of NK cell function by genetically coupled C-type lectin-like receptor/ligand pairs encoded in the human natural killer gene complex.. Front Immunol. 2013; 4:362. (Biology). View Reference
  2. Dennehy KM, Klimosch SN, Steinle A. Cutting edge: NKp80 uses an atypical hemi-ITAM to trigger NK cytotoxicity.. J Immunol. 2011; 186(2):657-61. (Clone-specific: Activation, Flow cytometry, Functional assay, Immunoprecipitation, Stimulation). View Reference
  3. Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A. NKp80 defines and stimulates a reactive subset of CD8 T cells.. Blood. 2009; 113(2):358-69. (Clone-specific: (Co)-stimulation, Flow cytometry, Functional assay). View Reference
  4. Welte S, Kuttruff S, Waldhauer I, Steinle A. Mutual activation of natural killer cells and monocytes mediated by NKp80-AICL interaction. Nat Immunol. 2006; 7(12):1334-1342. (Immunogen: Flow cytometry, Functional assay). View Reference
View All (4) View Less
566329 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.