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PE Mouse Anti-Human fMLP receptor
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse C57BL/6 IgG1, κ
Human fMLP receptor Transfected Cell Line
Flow cytometry (Routinely Tested)
20 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
556016 Rev. 5
Antibody Details
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The 5F1 monoclonal antibody specifically binds to the N-formyl-leucyl-phenylalanine receptor  (fMLP receptor), which is also known as, Formyl peptide receptor 1 (FPR1). This 55-70 kDa receptor is expressed on neutrophils, monocytes, macrophages, and liver parenchymal cells. It is composed of seven hydrophobic membrane spanning regions and is a member of the rhodopsin superfamily of G protein-coupled receptors. N-formyl peptides interact with fMLP receptors and induce neutrophil chemotaxis, phagocytosis, production of superoxide radicals and release of proteolytic enzymes. The binding of the 5F1 antibody to the fMLP receptor can be blocked by fMLP peptide.

556016 Rev. 5
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
556016 Rev.5
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Lala A, Sojar HT, De Nardin E. Expression and purification of recombinant human N-formyl-L-leucyl-L-phenylalanine (FMLP) receptor: generation of polyclonal antibody against FMLP receptor. Biochem Pharmacol. 1997; 54(3):381-390. (Biology). View Reference
  3. Sengeløv H, Boulay F, Kjeldsen L, Borregaard N. Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Biochem J. 1994; 299(2):473-479. (Biology). View Reference
556016 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.