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PE Mouse Anti-Human CD119
PE Mouse Anti-Human CD119

Expression of cell surface CD119  by human peripheral blood mononuclear cells. Human PBMC were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with PE Mouse Anti-Human CD119 (1 µg; Cat. No. 558937; filled histogram) or PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; open histogram). The histograms were generated from reanalyzed flow cytometric data files that were gated for cells that had the light-scattering characteristics of lymphocytes.

Expression of cell surface CD119  by human peripheral blood mononuclear cells. Human PBMC were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with PE Mouse Anti-Human CD119 (1 µg; Cat. No. 558937; filled histogram) or PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; open histogram). The histograms were generated from reanalyzed flow cytometric data files that were gated for cells that had the light-scattering characteristics of lymphocytes.

Product Details
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BD Pharmingen™
IFN-γ Receptor α; IFN-γRα; IFNGR1; IFNGR; IFN-gamma receptor 1/IFN-gamma-R1
Human (QC Testing)
Mouse IgG2b, κ
Human IFN-γRα
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397165
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Immunofluorescent staining and flow cytometric analysis: PE Mouse Anti-Human CD119 (Cat. No. 558937) can be used for the immunofluorescent staining (≤ 1 µg antibody/10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. An appropriate immunoglobulin isotype control is PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058).

Since GIR-94 is a non-neutralizing antibody, it can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the IFN-γ ligand is present. Based on our testing results,(data not shown) the presence of exogenous recombinant human IFN-γ at levels ≤ 50 ng/10[6] cells was insufficient to inhibit the binding of GIR-94 (at 0.06 µg mAb/1 million cells).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558937 Rev. 3
Antibody Details
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GIR-94

The GIR-94 antibody specifically recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα; aka, CD119).  The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses.  The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium.  The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα  purified from human placenta.  The GIR-94 is a non-neutralizing antibody.

558937 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558937 Rev.3
Citations & References
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Development References (4)

  1. Bach EA, Aguet M, Schreiber RD. The IFN gamma receptor: a paradigm for cytokine receptor signaling. Annu Rev Immunol. 1997; 15:563-591. (Biology). View Reference
  2. Greenlund AC, Schreiber RD, Goeddel DV, Pennica D. Interferon-gamma induces receptor dimerization in solution and on cells. J Biol Chem. 1997; 268(24):18103-18110. (Biology). View Reference
  3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:818-821.
  4. Sheehan KC, Calderon J, Schreiber RD. Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor. J Immunol. 1988; 140(12):4231-4237. (Immunogen). View Reference
View All (4) View Less
558937 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.