The RL73 monoclonal antibody specifically binds to mouse CD147. CD147 is type I membrane glycoprotein and member of the Ig superfamily. CD147 is also known as extracellular matrix metalloproteinase inducer (EMMPRIN) and gp42/basigin (BSG). The CD147 molecule is widely expressed on a variety of hemopoietic and non-hemopoietic cell types including thymocytes, lymphocytes, monocytes, granulocytes, erythroblasts and erythrocytes, endothelial cells and neoplasms. CD147 reportedly plays significant roles in the reproductive, nervous and immune systems and in tumor progression. In the immune system, CD147 can function as an adhesion molecule and signaling receptor that regulates leukocyte trafficking and immune and inflammatory responses.
The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.