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Biotin Mouse Anti-Rat IgG1/2a
Product Details
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BD Pharmingen™
IgG1/IgG2a; Ig gamma-1/2a; Immunoglobulin gamma 1/2a
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG2a
Pooled Rat IgG
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Biotin Mouse Anti-Rat IgG1/2a may be used as a secondary reagent in immunofluorescent staining. For immunohistochemical staining, we recommend the use of Biotin Mouse Anti-Rat IgG1/2a in our special formulation for immunohistochemistry, Cat. No. 550325.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  5. Please refer to to access safety data sheets (SDS).
  6. Please refer to for technical protocols.
553880 Rev. 13
Antibody Details
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The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.

553880 Rev. 13
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553880 Rev.13
Citations & References
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Development References (3)

  1. Badillo-Godinez O, Gutierrez-Xicotencatl L, Plett-Torres T, et al. Targeting of rotavirus VP6 to DEC-205 induces protection against the infection in mice.. Vaccine. 2015; 33(35):4228-37. (Clone-specific: Flow cytometry). View Reference
  2. Li Z, Kitagawa-Sakakida S, Horiguchi K, Tori M, Shirakura R. Antibody deposition in rat heart which develops transplant vasculopathy in (donor x recipient) F1 environment.. Surg Today. 2003; 33(7):509-17. (Clone-specific: Immunohistochemistry). View Reference
  3. Stermann A, Huebener N, Seidel D, et al. Targeting of MYCN by means of DNA vaccination is effective against neuroblastoma in mice.. Cancer Immunol Immunother. 2015; 64(10):1215-27. (Clone-specific: Immunohistochemistry). View Reference
553880 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.