Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No.561811) and with either Biotin Mouse IgM, κ Isotype Control (Cat. No. 553473; Left Plot) or Biotin Mouse Anti-Human CD57 antibody (Cat. No. 568064; Right Plot) at 1 µg/test. The cells were washed and counterstained with PE Strptavidin (Cat. No. 554061). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD57 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody (Cat. No.561811) and with either Biotin Mouse IgM, κ Isotype Control (Cat. No. 553473; Left Plot) or Biotin Mouse Anti-Human CD57 antibody (Cat. No. 568064; Right Plot) at 1 µg/test. The cells were washed and counterstained with PE Strptavidin (Cat. No. 554061). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD57 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
B3GAT1; Beta-1,3-glucuronyltransferase 1; HNK1; LEU7; NK-1; GLCATP; GLCUATP
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Streptavidin RUO
Size
0.5 mg
Cat No.
554061
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Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
APC Mouse Anti-Human CD3 RUO
Size
500 Tests
Cat No.
561811
Biotin Mouse IgM, κ Isotype Control RUO
Size
0.25 mg
Cat No.
553473
568064 Rev. 1
Antibody Details
NK-1
The NK-1 monoclonal antibody specifically reacts with a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein expressed on 7-35% of normal peripheral blood lymphocytes including a subset of natural killer cells, a subset of CD8-positive peripheral blood T cells, and on some neural tissues. CD57 is not expressed on granulocytes, platelets, red blood cells or thymocytes. The function of CD57 is still unclear, however, its expression on T-cell subets occurs in late immune responses.
568064 Rev. 1
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
568064 Rev.1
Citations & References
Development References (5)
-
Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981; 127(3):1024-1029. (Biology). View Reference
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Abo T, Cooper MD, Balch CM. Characterization of HNK-1+ (Leu-7) human lymphocytes. I. Two distinct phenotypes of human NK cells with different cytotoxic capability. J Immunol. 1982; 129(4):1752-1757. (Biology). View Reference
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McGarry RC, Helfand SL, Quarles RH, Roder JC. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature. 1983; 306(5941):376-378. (Biology). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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d'Angeac AD, Monier S, Pilling D, Travaglio-Encinoza A, Reme T, Salmon M. CD57+ T lymphocytes are derived from CD57- precursors by differentiation occurring in late immune responses. Eur J Immunol. 1994; 24(7):1503-1511. (Biology). View Reference
568064 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
